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Документ Antimicrobial properties of some porphyrin compounds against catheter related infections(Одеський національний університет імені І. І. Мечникова, 2018) Barlit, H.; Ovchinnikov, S.; Rusakova, Mariia Yu.; Русакова, Марія Юріївна; Русакова, Мария ЮрьевнаCentral venous access is commonly used in clinical practice for a wide spectrum of procedures. The main complications limiting use of central and peripheral catheters include catheter-related infections. The epidemic of microbial resistance to widely-used antibiotic groups seriously complicates treatment of CRI even in complex with systemic antibiotic therapy. In this paper we propose to consider porphyrin compounds as alternative antimicrobial agents for antimicrobial lock therapy. It was discovered antimicrobial action of porphyrin compounds against S. aureus and E. coli that was significantly enhanced in composition with EDTA as additional component of antimicrobial lock solutionДокумент Applying bioactive compounds of Pseudomonas spp. for medicine substance development(Одеський національний університет імені І. І. Мечникова, 2024) Marchenko, Mariia; Rusakova, Mariia Yu.; Русакова, Марія ЮріївнаInfectious diseases are still a worldwide important problem. This fact has led to the characterization of new biomarkers that would allow an early, fast and reliable diagnostic and targeted therapy. In this context, Pseudomonas aeruginosa can be considered one of the most threatening pathogens since it causes a wide range of infections. Antibiotic treatment is not trivial given the incidence of resistance processes and the fewer new antibiotics that are placed on the market. With this scenario, relevant quorum sensing (QS) molecules that regulate the secretion of virulence factors and biofilm formation can play an important role in diagnostic and therapeutic issues [Winstanley et al., 2009].Документ Influence of New N-Methylquinolinyl Porphyrins on Different Candida albicans Development Forms(2017) Rusakova, Mariia Yu.; Ruschak, Olga V.; Vodzinskyi, Serhii V.; Ishkov, Yurii V.; Русакова, Мария Юрьевна; Рущак, Ольга Васильевна; Водзинский, Сергей Валентинович; Ишков, Юрий Васильевич; Ішков, Юрій Васильович; Русакова, Марія ЮріївнаThe activity of new N-methylquinolinyl porphyrins against different morphological forms of Candida albicans (unattached, free-dividing cells, as well as structures formed in the process of biofilm maturation) was characterized. Modification of porphyrin molecules by N-methyl-6-quinolinyl increases the fungicidal effect on the yeast cells, in contrast to N-methyl-7-quinolinyl derivatives that are effective against hyphal structures. Candida albicans biofilm cells are more resistant to low concentrations of the studied compounds however the significant inhibition of the microbial community is in the presence of 1 μM.Документ Phenazine pigments production characteristics of pseudomonas bacteria(2015) Titlianov, O. O.; Rusakova, Mariia Yu.; Русакова, Марія Юріївна; Русакова, Мария ЮрьевнаPhenazines are low-molecular heterocyclic nitrogen-bearing compounds, the basis of which is phenazine-1-carboxylate. The largest variety of produced compounds is typical for Pseudomonas bacteria. The aim of this work was to compare phenazine spectra produced by several Pseudomonas species and their synthesis regulation. The work was carried out at Biotechnological Research and Training Center using P. aeruginosa (ATCC 10145, ATCC 15692, ATCC 10153, ATCC 9027), P. fluorescens ONU 303, P. chlororaphis (ONU 304, ONU 306).Документ Synthesis characteristics of siderophores of different types formed by pseudomonads under different cultivation conditions(Одеський національний університет імені І. І. Мечникова, 2020) Levchenko, V.; Rusakova, Mariia Yu.; Русакова, Марія Юріївна; Русакова, Мария ЮрьевнаThe aim of this work was to develop a method of obtaining siderophores and to study the influence of different cultivation conditions, in particular the composition of nutrient media, on the spectrum and level of their synthesis by some members of the Pseudomonas genus. Strains of Pseudomonas chlororaphis ONU 306, Pseudomonas fluorescens ATCC 13325 and Pseudomonas aeruginosa ATCC 10145 produced more intensely chelating compounds during cultivation in King B liquid medium. The corresponding values were higher than 9% for P. aeruginosa ATCC 10145) to 47% (for P. chlororaphis ONU 306). The spectrum of compounds of the siderophore series formed by the studied strains of pseudomonads mainly consisted of substances of hydroxamate and catechol type.Документ The anti-candida activity of new n-methylquinolinyl- porphyrins in vitro(Odessa I. I. Mechnikova National University, 2017) Ovchinnikov, S.; Vasyil'yev, M.; Rusakova, Mariia Yu.; Русакова, Мария Юрьевна; Русакова, Марія ЮріївнаThe activity of new N-methyl-quinolinyl-porphyrins against different morphological forms of Candida albicans (unattached, freedividing cells, as well as structures formed in the process of biofilm maturation) was characterized. Modification of porphyrin molecules by N-methyl-6-quinolinyl increases the fungicidal effect on the yeast cells, in contrast to N-methyl-7-quinolinyl derivatives that are effective against hyphal structures. Candida albicans biofilm cells are more resistant to low concentrations of the studied compounds.Документ The antibiotic influence on antagonistic activity of microorganisms that are the base of some probiotics(Одеський національний університет імені І. І. Мечникова, 2020) Lazar, A.; Sarova, N.; Rusakova, Mariia Yu.; Русакова, Марія Юріївна; Русакова, Мария ЮрьевнаThe work was devoted to the study of the strain interactions that isolated from probiotics with some opportunistic pathogens. In the co-cultivation of L. sporogenes and B. clausii probiotic strains, obtained from "Lactovit Forte" and "Enterogermina" preparations and strains of E. coli ATCC 25922 and S. aureus ATCC 25923, an antagonistic interaction was determined. The addition of 0.0313 mg/ml of ciprofloxacin enhanced the antagonistic interaction between probiotic strains, in particular B. clausii and cultures of E. coli ATCC 25922 and S. aureus ATCC 25923.Документ The Candida albicans and Pseudomonas aeruginosa interaction characteristics during the multispecies biofilm formation(Odessa I. I. Mechnikova National University, 2017) Rushchak, O. V.; Savitskaya, K.; Shpak, A.; Rusakova, Mariia Yu.; Русакова, Мария Юрьевна; Русакова, Марія ЮріївнаThe Candida albicans and Pseudomonas aeruginosa interaction characteristics during the multispecies biofilm formation were studied. At all formation stages the cultures of a microbial multispecies association influenced each other, manifesting an antagonistic form of interactions. The antagonism degree of the cultured microorganisms was not the same in different media.Документ The characteristics of Candida albicans biofilm formation in vitro(2015) Rushchak, O. V.; Rusakova, Mariia Yu.; Русакова, Марія Юріївна; Русакова, Марія ЮріївнаCandida albicans is a dimorphic fungus that exhibits a number of different morphological forms under different environmental conditions. During human candidiasis development this microorganism forms biofilms. C. albicans biofilms usually consist both yeast cells and hyphae covered with a thick exopolysaccharide layer. The C. albicans biofilm structure, in particular the component ratio, can be different depending on the substrate type: artificial medical devices, skin or mucous membranes. The aim of this work was to characterize the Candida albicans biofilm formation process under different conditions in vitro.Документ The characteristics of cellular dimorphism of some Candida albicans strains(Одеський національний університет імені І. І. Мечникова, 2020) Skalozub, D. V.; Rusakova, Mariia Yu.; Русакова, Марія Юріївна; Русакова, Мария ЮрьевнаThe study revealed clear morphological differences for both colonies of C. albicans and at the cellular level. The data obtained indicate that strains of C. albicans, which are characterized by the ability to form hyphae, form on the surface of Spider agar colonies with wrinkles on the surface. Smooth colonies of C. albicans, in most cases with a matte surface, consist of yeast-like cells.Документ The characteristics of microbial interaction between some representatives of human microbiota and probiotic strains(Одеський національний університет імені І. І. Мечникова, 2020) Sarova, N.; Lazar, A.; Rusakova, Mariia Yu.; Русакова, Марія Юріївна; Русакова, Мария ЮрьевнаThe work was dedicated to the study of the interaction of strains isolated from probiotics, with some opportunistic microorganisms. It has been established that between L. acidophilus, L. sporogenes, B. clausii and the opportunistic strains (Candida albicans ATCC 18804, Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923) antagonistic, neutral and mutual interactions develop. Over time, the form of interaction may change. The most effective, stable antimicrobial effect caused by the biologically active exometabolite production was determined for L. acidophilus to all investigated test microorganisms.Документ The characteristics of phenazine pigments that produced by some Pseudomonas strains(Одеський національний університет імені І. І. Мечникова, 2020) Levchenko, V.; Chukaeva, J.; Rusakova, Mariia Yu.; Русакова, Марія Юріївна; Русакова, Мария ЮрьевнаAt present time phenazine compounds produced by Pseudomonas bacteria are applied in many industries. These substances are used in the biotechnology, including agricultural production. The aim of this work was to study the effect of the nutrient composition on the intensity of accumulation of phenazine compounds by pseudomonad cultures. The dependence of the intensity of accumulation of phenazine antibiotics on the composition of the nutrient medium, in particularly the presence of metal cations in it was detected. It can be assumed that the phenazine derivative synthesis is strain specific process. It was also noted that the intensity of the production of these compounds did not correspond to the rate of cell biomass accumulation by the studied Pseudomonas strains.Документ The characteristics of Pseudomonas bacteria in the plant rhizosphere(Odesa I. I. Mechnikov National University, 2016) Tyagniryadno, L.; Rusakova, Mariia Yu.; Русакова, Мария Юрьевна; Русакова, Марія ЮріївнаAntagonism is one of the main symbiotic relationship type in which one participant (antagonist) completely inhibits or slows the growth of another (Bukharin O., 2006). In vivo the most antagonistic relationships are in plant rhizosphere microbiota (Xing-Feng Huang, 2014). All of them are in close relations with plant roots that resulted in influence on plant growth and productivity in generally. Specific microorganisms are able to protect the plant either directly or indirectly against pathogens, but also they largely influence on the rest of the microbial community (Kamble K.D., 2015). The largest number of plant rhizosphere microbiota members includes Pseudomonas bacteria that are regarded as plant-growth-promoting rhizobacteria (PGPR) (Sivasalkhi S., 2014).Документ The culture condition effects on the Candida albicans biofilm formation process(Odesa I. I. Mechnikov National University, 2016) Rushchak, O. V.; Rusakova, Mariia Yu.; Русакова, Марія Юріївна; Русакова, Мария ЮрьевнаCandida albicans is a polymorphic microorganism existed as either true yeast fungi or as the hyphae and pseudohyphae forms. This microorganism is a part of the normal microbiota. But during human violations candidiasis arise and the biofilm development begins. The C. albicans biofilm structure can be different, depending on their formation surfaces (Zhu W., 2010).Документ The influence of some synthetic porphyrins on the culture of Candida albicans(Odesa I. I. Mechnikov National University, 2016) Ovchinnikov, S.; Vasyil'yev, M.; Rusakova, Mariia Yu.; Русакова, Марія Юріївна; Русакова, Мария ЮрьевнаPorphyrins are macroheterocyclic compounds, containing conjugated system, which is based on four pyrrole rings linked by four methine groups in α-positions. Two classes of porphyrins, natural and synthetic, can be distinguished on the basis of their origin and structure (Soman R., 2015).Документ The mechanisms of microbial interaction during the multispecies association formation(Одеський національний університет імені І. І. Мечникова, 2018) Savitskaya, K.; Vasyil'yev, M.; Rusakova, Mariia Yu.; Русакова, Мария Юрьевна; Русакова, Марія ЮріївнаThe Candida albicans, Staphylococcus aureus and Pseudomonas aeruginosa interaction characteristics during the multispecies biofilm formation were studied. At all formation stages the cultures of a microbial multispecies association influenced each other, manifesting an antagonistic form of interactions. The antagonism degree of the cultured microorganisms was not the same in different media.Документ The production phenazine compounds by some stems of Pseudomonas aeruginosa(Одеський національний університет імені І. І. Мечникова, 2018) Shiryaeva, Yu.; Rusakova, Mariia Yu.; Русакова, Мария Юрьевна; Русакова, Марія ЮріївнаThe work was carried out in the Biotechnology Research and Training Center I. I. Mechnikov National University. The various Pseudomonas phenazine synthesis intensity and the compound production dependence on the forms of microbial existence were compared.Документ The rhizosphere microbiota influence on the Fusarium fungi(Odessa I. I. Mechnikova National University, 2017) Palamarchuk, A.; Rusakova, Mariia Yu.; Русакова, Марія Юріївна; Русакова, Мария ЮрьевнаThe influence of bacterial cultures (B. megaterium ONU 500, B. subtilis ONU 410, P. fluorescens ONU 303, P. chlororaphis ONU 305) on the Fusarium (F. oxysporum, F. graminearum) growth was determined by the methods of agar block and well co-cultivation. The most expressed anti-fusarium effect was characterized for P. chlororaphis ONU 304.Документ Активність ізатинового гідразида феноксиоцтової кислоти щодо деяких штамів FUSARIUM SPP.(Одеський національний університет імені І.І. Мечникова=Odesa National Mechnykov University, 2010) Русакова, Марія Юріївна; Галкін, Борис Миколайович; Вострова, Л. М.; Філіпова, Тетяна Олегівна; Гренадьорова, М. В.; Русакова, Мария Юрьевна; Галкин, Борис Николаевич; Вострова, Л. Н.; Филиппова, Татьяна Олеговна; Гренадёрова, М. В.; Rusakova, Mariia Yu.; Galkin, Borys M.; Vostrova, L. M.; Filipova, Tetiana O.; Grenaderova, M. V.Вивчено протифузаріозну активність 2,3-індоліндіонового гідразида 2,4-дихлорфеноксиоцтової кислоти. Максимальний рівень дії на штами мікроміцетів спостерігався для 1,0 мМ даної сполуки. Визначено, що фунгіцидна активність досліджуваного гідразида визначається наявністю ізатинового (2,3-індоліндіонового) компоненту молекули.Документ Антимікробна дія фенотіазинованих сполук(Одеський національний університет імені І.І. Мечникова=Odesa National Mechnykov University, 2011) Русакова, Марія Юріївна; Галкін, Борис Миколайович; Соболєва, С. Г.; Філіпова, Тетяна Олегівна; Русакова, Мария Юрьевна; Галкин, Борис Николаевич; Соболева, С. Г.; Филиппова, Татьяна Олеговна; Rusakova, Mariia Yu.; Galkin, Borys M.; Soboleva, S.G.; Filipova, Tetiana O.В роботі вивчена дія фенотіазинових сполук на Staphylococcus aureus та Escherichia coli. Показано, що ефективність досліджуваних речовин щодо E. coli після попередньої світлової активації перевищує активність метиленового синього. S. aureus виявив стійкість до впливу похідних фенотіазину, як у темнових умовах, так і за фотоінактивації.