Preference of molecular markers for detecting allelic variants of gliadins

Вантажиться...
Ескіз
Дата
2023
Науковий керівник
Укладач
Редактор
Назва журналу
ISSN
E-ISSN
Назва тому
Видавець
Одеський національний університет імені І. І. Мечникова
Анотація
The main method of studying allelic variants of gliadins is electrophoresis in acidic PAGE, which actually shows the phenotype determined by the set of genes of the gliadin-encoding locus. This method is difficult to use, because peptides encoded by all gliadin-encoding loci are present on one lane of the gel and may overlap. Therefore, there is a need for molecular markers that would allow the determination of allelic variants of gliadins using PCR. This will help to use gliadins for marker-asissted selection, to identify allelic variants of gliadins even before obtaining grain suitable for electrophoresis of storage proteins. In this regard, the aim of study was to analyze polymorphism of Gli‑1 loci using allele-specific molecular markers and microsatellites and find suitable molecular markers for detecting allelic variants of gliadins by PCR method.
Опис
Ключові слова
molecular markers, allelic variants, gliadins
Бібліографічний опис
Вісник Одеського національного університету = Odesa National University Herald
DOI
ORCID:
УДК