Гістоморфометрична оцінка змін структурних компонентів кістки у щурів з хронічним холестазом при корекції комплексом із гепатопротектора та сорбенту

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dc.contributor.authorРижко, Ірина Леонідівна
dc.contributor.authorКосенко, Тетяна Василівна
dc.contributor.authorМакаренко, Ольга Анатоліївна
dc.contributor.authorRyzhko, Iryna L.
dc.contributor.authorKosenko, Tetiana V.
dc.contributor.authorMakarenko, Olga A.
dc.date.accessioned2024-03-12T12:10:44Z
dc.date.available2024-03-12T12:10:44Z
dc.date.issued2023
dc.description.abstractПроведено гістоморфометричну оцінку змін структурних компонентів стегнової кістки у щурів із модельованим хронічним холестазом та при корекції комплексом, який складався з гепатопротектора та сорбенту. Показано, що хронічний холестаз призводить до виражених патологічних змін основних компонентів кістки, а застосування комплексу ефективно усуває встановлені порушення, завдяки компонентам гепатопротектора (лецитину і кверцетину) та сорбенту (сорбент та джерело макро- і мікроелементів).uk_UA
dc.description.abstractProblem. The disruption of the gastric duct in cholestasis causes insufficient absorption in the small intestine of macro- and microelements and fat-rich vitamins (A, D, E, K), which are necessary for healthy processes, and remodeling of bone tissue. To reduce pathological changes in bone tissue diseases of the hepatobiliary system in rats statement in cholestasis, need a comprehensive approach [6]. In the core of the preventive complex one can see a hepatoprotector, which, due to its main components (lecithin, quercetin), possesses the liver protecting properties, as well as has anti-inflammatory and bile-diluting effects, and a sorbent. Aim. To conduct a histomorphometric research of the femur in rats with chronic cholestasis and evaluate the effectiveness of the prophylactic complex. Methods. The follow-up study was carried out on male rats, divided into three groups: 1st group – intact, 2nd group – rats with simulated chronic cholestasis, 3rd group – rats, who, in parallel with the development of chronic cholestasis pathology, carried out prevention. Cholestasis in rats was modeled by ligation of the biliary duct under thiopental anesthesia [9]. A hepatoprotector at a dose of 500 mg/kg and a sorbent at a dose of 1 g/kg were administered to group 3 of the animals once over a period of 4 months. Histological analysis and photography of sections of the stegnus cyst were carried out using an additional light microscope and a camera for an Olympus LC30 microscope (Japan). Morphometric studies of rat femur were carried out using the following parameters: volume (%) of the articular epiphyseal cartilage, volume (%) of the cartilage, volume (%) of the cerebrum and the number of osteoblasts per the particular area. The main results. Certain histological changes in the epiphysis of the femoral bone were found in rats with chronic cholestasis, namely, noticeable delamination of the periosteum was noted. In the deep layers of the cartilage, a decrease in the number of isogenic groups was noted, in addition, they contained a smaller number of chondrocytes. Desolation of individual lacunae, a decrease in the number of osteoblasts, and thinning of bone trabeculae were also observed, indicating certain pathological changes in bone tissue. Analysis of the morphometric parameters of the femur in rats with chronic cholestasis revealed a decrease in the specific volume of the cartilage of the articular surfaces by 25.8%, a decrease in the specific volume of the bone marrow and the number of osteoblasts by 14.4% and 39.4%, respectively, and as well as an increase of 33.1% in the specific volume of bone beams in relation to the control group. The introduction of the prophylactic complex contributed to a significant increase in the specific volume of the cartilage of the articular surfaces – by 17.2%, the specific volume of bone marrow by 42.9%, the average number of osteoblasts by 34.9%, and a decrease in the specific volume of bone beams by 31 .7% relative to the group of animals with chronic cholestasis. That is, the use of a prophylactic complex, which consisted of a hepatoprotector and a sorbent, effectively eliminated pathological changes in bone tissue that arose against the background of the development of chronic cholestasis. Conclusions. The formation of chronic cholestasis leads to pathological changes in the cartilaginous tissue of the epiphysis of the femur of the animals under study, and it itself changes the volume of cartilage of the subglobular surfaces and causes destruction of the main components in bone tissue (reduces the volume of bone marrow and the number of osteoblasts at the background of the growth the volume of bone beams). The effectiveness of prophylactic in the elimination of pathological changes in bone tissue at the background of chronic cholestasis has manifested itself as a hepatoprotective, antioxidant, anti-inflammatory and anti-inflammatory hepatoprotector and those combined with a sorbent.
dc.identifierУДК 616–018: 616.71:59.085+616.36–008.811.6+615.276
dc.identifier.citationВісник Одеського національного університету = Odesa National University Heralduk_UA
dc.identifier.doihttps://doi.org/10.18524/2077–1746.2023.2(53).293329
dc.identifier.urihttps://dspace.onu.edu.ua/handle/123456789/37833
dc.language.isoukuk_UA
dc.publisherОдеський національний університет імені І. І. Мечниковаuk_UA
dc.relation.ispartofseries;Т. 28, вип. 2(53).
dc.subjectщуриuk_UA
dc.subjectхронічний холестазuk_UA
dc.subjectстегнова кісткаuk_UA
dc.subjectгепатопротекторuk_UA
dc.subjectсорбентuk_UA
dc.subjectморфометричний аналізuk_UA
dc.subjectгістологічне дослідженняuk_UA
dc.subjectratsuk_UA
dc.subjectchronic cholestasisuk_UA
dc.subjectfemuruk_UA
dc.subjecthepatoprotectoruk_UA
dc.subjectsorbentuk_UA
dc.subjectmorphometric analysisuk_UA
dc.subjecthistological researchuk_UA
dc.titleГістоморфометрична оцінка змін структурних компонентів кістки у щурів з хронічним холестазом при корекції комплексом із гепатопротектора та сорбентуuk_UA
dc.title.alternativeHistomorphometric assessment of changes the structural components of the bone in rats with chronic cholestasis with correction of the complex from hepatoprotector and sorbentuk_UA
dc.typeArticleuk_UA
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