Дослідження генотоксичного впливу гербіциду «Федерал» на модельному об’єкті Danio rerio, Hamilton, 1822. Повідомлення 1. Вплив високих концентрацій
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Файли
Дата
2024
Науковий керівник
Укладач
Редактор
Назва журналу
ISSN
2077-1746
E-ISSN
2415-3125
Назва тому
Видавець
Одеський національний університет імені І. І. Мечникова
Анотація
Досліджували дію гербіцидного препарату «Федерал» у концентраціях 5, 10 та 15 мг/л на модельному об’єкті Danio rerio в умовах in vivo. Виявлено достовірний вплив досліджуваних концентрацій препарату на збільшення частоти еритроцитів з мікроядрами. Генотоксичний вплив посилювався із збільшенням тривалості обробки до 96 годин. Подальше збільшення тривалості обробки не вплинуло суттєво на збільшення частоти клітин з мікроядрами.
Problem. More than 60% of all pesticides used in agriculture are herbicides. Glyphosate is the most widely used herbicide in the world. Questions regarding its safety, possible side effects and influence on other organisms are controversial and are widely discussed. Aim. The purpose of the presented work is to study the effect of high concentrations of the herbicide preparation based on glyphosate “Federal” on the test object Danio rerio, Hamilton, 1822. High concentrations are understood to be those that far exceed the MPC in the aquatic environment. Methods. The effect of the drug “Federal” on the frequency of erythrocytes with micronuclei when treated for 48, 96 and 144 hours at a concentration of 5, 10 and 15 mg/l (in terms of glyphosate) was studied on the test object Danio rerio. Intact individuals were used as a negative control, and sulfonamide at a concentration of 20 mg/l was used as a positive control for 48, 96 and 144 hours. Main results. When treated with sulfonamide, the frequency of cells with micronuclei significantly increased. All variants of treatment with the drug “Federal” showed significant differences from the negative control in the frequency of erythrocytes with micronuclei. For 48 hours of treatment with the drug “Federal” at any concentration, the frequency of cells with micronuclei was significantly lower than in the positive control. Both at 96-hour and at 144-hour herbicide treatment in concentrations of 5 and 10 mg/l, significant differences from the positive control remained. However, when treated with the drug with a concentration of 15 mg/l, no significant differences from the positive control were found during both periods of a long-term treatment. The analysis of the influence of concentrations of the drug “Federal” on the frequency of micronuclei during 48 hours of treatment revealed significant differences only in concentrations of 15 mg/l from other treatment options. As treatment duration increased, these differences persisted between treatment with a concentration of 5 mg/l and other treatment options. For all applied concentrations of the drug, the frequency of cells with micronuclei significantly increased with an increase in the duration of the treatment from 48 to 96 hours. Further increase in trivalency of processing did not significantly increase the frequency of erythrocytes from micronuclei. Conclusions. The effect of the drug “Federal” when using the micronucleus test showed a reliable effect of the studied concentrations on increasing the frequency of erythrocytes with micronuclei. The genotoxic effect increased with increasing duration of the treatment up to 96 hours. Further increase in the treatment duration did not significantly affect the increase in the frequency of cells with micronuclei.
Problem. More than 60% of all pesticides used in agriculture are herbicides. Glyphosate is the most widely used herbicide in the world. Questions regarding its safety, possible side effects and influence on other organisms are controversial and are widely discussed. Aim. The purpose of the presented work is to study the effect of high concentrations of the herbicide preparation based on glyphosate “Federal” on the test object Danio rerio, Hamilton, 1822. High concentrations are understood to be those that far exceed the MPC in the aquatic environment. Methods. The effect of the drug “Federal” on the frequency of erythrocytes with micronuclei when treated for 48, 96 and 144 hours at a concentration of 5, 10 and 15 mg/l (in terms of glyphosate) was studied on the test object Danio rerio. Intact individuals were used as a negative control, and sulfonamide at a concentration of 20 mg/l was used as a positive control for 48, 96 and 144 hours. Main results. When treated with sulfonamide, the frequency of cells with micronuclei significantly increased. All variants of treatment with the drug “Federal” showed significant differences from the negative control in the frequency of erythrocytes with micronuclei. For 48 hours of treatment with the drug “Federal” at any concentration, the frequency of cells with micronuclei was significantly lower than in the positive control. Both at 96-hour and at 144-hour herbicide treatment in concentrations of 5 and 10 mg/l, significant differences from the positive control remained. However, when treated with the drug with a concentration of 15 mg/l, no significant differences from the positive control were found during both periods of a long-term treatment. The analysis of the influence of concentrations of the drug “Federal” on the frequency of micronuclei during 48 hours of treatment revealed significant differences only in concentrations of 15 mg/l from other treatment options. As treatment duration increased, these differences persisted between treatment with a concentration of 5 mg/l and other treatment options. For all applied concentrations of the drug, the frequency of cells with micronuclei significantly increased with an increase in the duration of the treatment from 48 to 96 hours. Further increase in trivalency of processing did not significantly increase the frequency of erythrocytes from micronuclei. Conclusions. The effect of the drug “Federal” when using the micronucleus test showed a reliable effect of the studied concentrations on increasing the frequency of erythrocytes with micronuclei. The genotoxic effect increased with increasing duration of the treatment up to 96 hours. Further increase in the treatment duration did not significantly affect the increase in the frequency of cells with micronuclei.
Опис
Ключові слова
гліфосат, дикамба, мікроядерний тест, Danio rerio, glyphosate, dicamba, micronucleus test
Бібліографічний опис
Кузнецов М. К. Дослідження генотоксичного впливу гербіциду «Федерал» на модельному об’єкті Danio rerio, Hamilton, 1822. Повідомлення 1. Вплив високих концентрацій // М. К. Кузнецов, О. Л. Січняк // Вісник Одеського національного університету. Біологія. – 2024. – Т. 29, вип. 1(54). – С. 7–18.
УДК
575:574.24:574.524:576.08