Біотехнологія очищення навколишнього середовища від важко окислювальних циклічних сполук
Ескіз недоступний
Дата
2018
Науковий керівник
Укладач
Редактор
Назва журналу
ISSN
E-ISSN
Назва тому
Видавець
Одеський національний університет імені І. І. Мечникова
Анотація
Видiлeний з нaфтoзaбрyднeнoгo ґрyнтy штaм бaктeрiй iдeнтифiкoвaний зa жирнoкислoтним склaдoм як Pseudomonas fluorescens 17. Показана його здатність до деградації важко окислювальної циклічної сполуки N-цетилпіридинію бромистого та вуглеводнів нафти. Розроблена біотехнологія очищення навколишнього середовища від важкоокислювальних циклічних сполук з застосуванням бактерій роду Рseudomonas. Лабораторні випробування показали ефективність її використання для очистки ґрунту від вуглеводнів нафти.
The derivative of the naftozabrydnnego grains of the bacterial strains antigenic with a fatty acid glass like Pseudomonas fluorescens 17. It shows its ability to degrade the heavy oxidizing cyclic compound of N-cetylpyridinium bromide and hydrocarbon oils. Biotechnology was developed for purifying the environment from heavy-oxidising cyclic compounds using bacteria of the genus Pseudomonas. Laboratory tests have shown the effectiveness of its use to clean the soil from hydrocarbon oil.
To create a biological preparation, a selection of Lactobacillus plantarum strains isolated from fermented plant products from Ukraine and France, based on the adhesive properties and capability to form biofilms has been conducted. It was shown that many of the strains isolated in France exhibited an average level of the biofilm formation on roots of Lepidium sativum, and among the strains isolated in Ukraine, almost equal percentages of strains with medium and low biofilm formation were found. On aerial parts of Lepidium sativum all strains showed high levels of biofilms formation. Inactivation of adhesins showed that attachment to Lepidium sativum roots mainly depended on the S-layer proteins. All strains were characterized by a clear antagonistic activity against phytopathogens R. radiobacter and R. vitis. Competitive adhesion on glass with a strain of L. plantarum ONU 355 showed complete inhibition of attachment of R. radiobacter pJZ pathogen cells. Competentive adhesion on tomato roots showed that phytopathogen was eliminated from the root after adding lactobacilli, but it can insert into lactobacilli biofilm after lactobacilli attachment.
The derivative of the naftozabrydnnego grains of the bacterial strains antigenic with a fatty acid glass like Pseudomonas fluorescens 17. It shows its ability to degrade the heavy oxidizing cyclic compound of N-cetylpyridinium bromide and hydrocarbon oils. Biotechnology was developed for purifying the environment from heavy-oxidising cyclic compounds using bacteria of the genus Pseudomonas. Laboratory tests have shown the effectiveness of its use to clean the soil from hydrocarbon oil.
To create a biological preparation, a selection of Lactobacillus plantarum strains isolated from fermented plant products from Ukraine and France, based on the adhesive properties and capability to form biofilms has been conducted. It was shown that many of the strains isolated in France exhibited an average level of the biofilm formation on roots of Lepidium sativum, and among the strains isolated in Ukraine, almost equal percentages of strains with medium and low biofilm formation were found. On aerial parts of Lepidium sativum all strains showed high levels of biofilms formation. Inactivation of adhesins showed that attachment to Lepidium sativum roots mainly depended on the S-layer proteins. All strains were characterized by a clear antagonistic activity against phytopathogens R. radiobacter and R. vitis. Competitive adhesion on glass with a strain of L. plantarum ONU 355 showed complete inhibition of attachment of R. radiobacter pJZ pathogen cells. Competentive adhesion on tomato roots showed that phytopathogen was eliminated from the root after adding lactobacilli, but it can insert into lactobacilli biofilm after lactobacilli attachment.
Опис
Ключові слова
162 біотехнології та біоінженерія, біотехнологія, важкоокислювальні циклічні сполуки, бaктeрiї-дeстрyктoри рoдy Pseudomonas, вуглеводні нафти, oчистка ґрyнтy, поверхнево-активні речовини, biotechnology, heavy oxidizing cyclic compounds, bactericidal properties Pseudomonas, hydrocarbon oils, purification of soil, surfactants
Бібліографічний опис
Самофалов, Михайло Олександрович. Біотехнологія очищення навколишнього середовища від важко окислювальних циклічних сполук = Biotechnology purification of environment from hard oxidizing cyclic compounds : Дипломна робота магістра / М. О. Самофалов; наук. кер. Т. В. Гудзенко; ОНУ ім. І.І. Мечникова, Біол. ф-т, Каф. мікробіології, вірусології та біотехнології. – Одеса, 2018. – 60 с.