Methodology for accelerated monitoring and assurance of sanitary quality and food safety
Вантажиться...
Дата
2017
Науковий керівник
Укладач
Редактор
Назва журналу
ISSN
E-ISSN
Назва тому
Видавець
Анотація
Introduction. The microbiological criteria that ensure
sanitary quality and safety of food products, methods for
identifying regulated microorganisms, and the urgency of
accelerated microbiological control of food safety are
characterized.
Materials and methods. The methodology for assessing the
safety of products and the classical and accelerated methods for
determining regulated microbiological indicators that identify
the presence of heat-resistant pathogens of food diseases are
studied. Analytical studies are based on modern literary sources
and some of own results.
Results and discussion. The characteristics of
microbiological criteria and requirements for microbiological
safety of food products were given. Analysis of modern
requirements for the sanitary safety of food has shown the need
for microbiological control for the presence of heat-resistant
microorganisms, which are potential pathogens of foodborne
diseases. Microbial species traditionally the main assessment of
their health status such as Clostridium botulinum, Bacillus
cereus, Clostridium perfringens, Staphylococcus aureus were
given. Characteristics of the phenotypes and genotypic
properties of criterial microorganisms – potential causative
agents of foodborne infections and poisonings are given. The
study methodology and methods of control of regulated
microorganisms showed failure and inaccuracy of their
phenotypic diagnosis due to the similarity of morphological and
tinctorial properties within the individual groups, the variability
of a number of biochemical parameters, weak antigenicity for the
immunological diagnosis, the advent of new metabolic features
associated with the ability to synthesize genes toxicity by
microorganisms, which were traditionally considered to be nonpathogenic,
labor-consuming and durable analysis. Genotypic
diagnostics of microorganisms using modern molecular genetic
methods and methodologies, in contrast to the phenotypic one,
ensures the accuracy of identification, the ability to monitor and
predict the behavior of pathogens of foodborne infections and
toxic infections in products in assessing microbiological risk,
allows accelerated microbiological control of food safety, taking
into account their specific features of composition and
properties, is a reliable method of sanitary control.
Conclusions. Molecular genetic diagnosis of pathogens is a
promising accelerated method for determining food safety and is
relevant especially in the Ukrainian region.
Опис
Ключові слова
food, sanitary, safety, phenotypic, genotypic, control, PCR
Бібліографічний опис
Ukrainian Food Journal