Перегляд за Автор "Shutylo, M. V."

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    Antimycotic activity of the isolates of lactobacteria from water and mussels of Black Sea
    (Одеський національний університет імені І. І. Мечникова, 2023) Merlich, Andrii H.; Kimurzhyi, I. I.; Kovalchuk, R. R.; Shutylo, M. V.; Ivanytsia, Volodymyr O.; Мерліч, Андрій Геннадійович; Кімуржий, І. І.; Ковальчук, Р. Р.; Шутило, М. В.; Іваниця, Володимир Олексійович
    Determination of the presence of antimycotic activity and its level in thirteen lactobacteria isolates from Black Sea water and mussels. Methods. Antimycotic activity of lactic acid bacteria (LAB) isolates was tested by agar diffusion method in 24-well plates. To determine character of action the plates were incubated for 7 days at 25 °C. Results. For the first time antimycotic activity of marine LAB from Odesa coast was estimated. The majority of the tested isolates of lactobateria exhibited antimycotic activity of high level completely inhibiting (in 100%) as mycelium growth as well as spore formation of Penicillium expansum UKM F-575 and Aspergillus niger UKM F-16706 on second day of the study. Four isolates (M.5.1, M.5.2, M.7.1, M.7.2) showed lower antimycotic activity (from 0 to 75%). Eight LAB isolates from seawater (W.1.1, W.1.2, W.1.3, W.1.4, W.1.5, W.1.дк, W.2.3, W.2.4) and one isolate from mussel liquor (M4.1) completely inhibited mycelial growth and sporulation of P. expansum UКМ F-575 and A. niger UКМ F-16706 even within seven days indicating fungicidal character of action. Conclusions. The most of tested LAB isolates from Black Sea exhibited high antimycotic activity against P. expansum UКМ F-575 and A. niger UКМ F-16706 indicator strains. The most active lactobacteria isolates were W.1.1, W.1.2, W.1.3, W.1.4, W.1.5, W.1.дк, W.2.3, W.2.4 isolated from Black Sea water and M4.1 – from mussel liquor. The character of the revealed antimycotic activity was determined as fungicidal.
  • Ескіз
    Документ
    Characterization of lactobacteria from the Black Sea water and mussels with the potential to produce antibacterial compounds
    (Одеський національний університет імені І. І. Мечникова, 2024) Merlich, Andrii H.; Kalnytska, O. Iu.; Shutylo, M. V.; Kovtun, Oleh O.; Ivanytsia, Volodymyr O.; Мерліч, Андрій Геннадійович; Кальницька, Олександра Юріївна; Шутило, Марина Василівна; Ковтун, Олег Олексійович; Іваниця, Володимир Олексійович
    Aim. Characterization of lactic acid bacteria (LAB) from water and mussels of the Black Sea with the potential to produce antibacterial compounds. Methods. The isolation of lactobacteria from water and mussels, their enumeration were performed by standard microbiological methods. Determination of their morphological, tinctorial, cultural, and biochemical characteristics were performed by Gram staining with immersion microscopy, describing the growth in liquid MRS media with and without 6.5% NaCl, catalase test. Genus-specific classic PCR was used for identification of isolated bacteria. To study the antagonistic interactions of the strains the perpendicular streak method was used. Results. In water of the Black Sea from Odesa bay, collected in winter period, 2×10–4.8×102 CFU/ mL of lactobacteria were found, while only in three mussels from seven (42.9%) LAB were detected (1.7×10±0.7 CFU/mL). Thirteen new strains of lactobacteria were isolated and characterized – eight strains from water and five from mussels. Among them, nine strains were identified as Enterococcus sp., one strain – as Lactobacillus sp., and three LAB strains remained unidentified. The new strains Enterococcus W1.1, Enterococcus W1.2, Enterococcus W1.3, Enterococcus W2.3, and Enterococcus М7.1 exhibited antagonistic activity against other closely related strains and Lactobacillus sakei subsp. sakei JCM1157. Conclusions. The Black Sea water and mussels tissue liquor in winter period contain 2×10–4.8×102 CFU/mL and 1.7×10±0.7 CFU/mL of LAB, respectively. The strains Enterococcus W1.1, Enterococcus W1.2, Enterococcus W1.3, Enterococcus W2.3, and Enterococcus М7.1 are potential producers of antibacterial compounds.