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https://dspace.onu.edu.ua/handle/123456789/2062

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Перегляд Статті та доповіді БФ за Автор "Andriuschenko, O."

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    The influence of secondary exometabolites of Streptomyces ambofaciens ONU 561 on phytopathogenic micromycetes
    (Odesa I. I. Mechnikov National University, 2024) Andriuschenko, O.; Lisiutin G.; Strashnova, Iryna V.; Андрющенко, О.; Лісютін, Г.; Страшнова, Ірина Валентинівна
    The development of microbial preparations based on antagonistically active strains of microorganisms is a very promising direction in the fight against pathogens of fusarium head and grain of wheat and barley. The aim of work was to study the effect of extracted secondary exometabolites of Streptomyces ambofaciens ONU 561 on phytopathogenic micromycetes. Extracted secondary exometabolites of strain S. ambofaciens ONU 561 in concentrations of 25 µg/ml, 50 µg/ml, 100 µg/ml, 250 µg/ml, 500 µg/ml, 1000 µg/ml were applied in 5 µl on sterile discs. To study the effect of extracted exometabolites on collection strains of micromycetes and Fusarium oxysporum strains isolated from infected wheat and barley plants, Petri dishes with Sabouraud's agar medium with glucose were inoculated. After that, disks soaked extracted exometabolites, in working concentrations, were placed on the inoculations. The results were recorded every day for 10 days, measuring the sizes of the zones of no growth of micromycete strains around the disks. Both collection strains of micromycetes and phytopathogenic strains of F. oxysporum were sensitive to extracted secondary exometabolites of S. ambofaciens ONU 561. The zones of no growth of micromycete strains varied widely and depended on the micromycete strain, the exometabolite concentration and the term of registration of the results. The minimum inhibitory concentrations (MIC) of the extracted secondary exometabolites, which inhibited the growth of the micromycete collection strains, were 100 – 250 µg/ml, the phytopathogenic F. oxysporum strains were higher and were at least 250 µg/ml. The presence of compounds with antimycotic activity in the pool of extracted secondary exometabolites allows to recommend strain S. ambofaciens ONU 561 for a more detailed study of the possibility of its use to create antimycotic preparations for agriculture.