The use of bacteriophage T7 in biotechnology for food protection from Escherichia coli
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Файли
Дата
2016
Автори
Науковий керівник
Укладач
Редактор
Назва журналу
ISSN
E-ISSN
Назва тому
Видавець
Odesa I. I. Mechnikov National University
Анотація
In the developing countries, the lack of clean drinking water and food is a
critical health problem. The World Health Organization (WHO) estimated that 1.5
million children die from diarrheal diseases every year. 88% of such diseases were
caused by unsafe drinking water and inadequate sanitation of food products.
Traditional microbiological cultural methods for the detection of bacterial
contamination require 2 days or more. Verification of species identity requires
additional morphological testing or further subcultivation in differential media.
While the rapid detection of these cells it is very important to prevent the risk of
foodborne diarrheal diseases. Other culture-independent methods such as those
based on polymerase chain reaction (PCR) are also used for identifying bacteria in
water and food samples. These DNA-targeted methods detect the presence of
genes specific to bacterial species or strains and they offer good sensitivity and
speed using minimal number of sample. However, PCR is unable to discriminate
viable bacterial cells from dead. Such false positive results can only be verified
through microbiological or other methods.
Опис
Ключові слова
Escherichia coli, bacteriophage T7, microorganisms
Бібліографічний опис
"Molecular microbiology and biotechnology", International scientific conference": abstracts, Odessa, Ukraine, June 21-23, 2016 / Odessa I.I. Mechnikov national univ. ; Chairman: V. O. Ivanytsia [et al.] . – Odessa, 2016.