Metagenomic 16s rRNA investigation of microbial communities in the Black Sea estuaries in South-West of Ukraine
Дата
2016
Науковий керівник
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Анотація
The Black Sea estuaries represent interfaces of the sea
and river environments. Microorganisms that inhabit
estuarine water play an integral role in all biochemical
processes that occur there and form unique ecosystems.
There are many estuaries located in the Southern-Western
part of Ukraine and some of them are already separated
from the sea. The aim of this research was to determine
the composition of microbial communities in the
Khadzhibey, Dniester and Sukhyi estuaries by metagenomic
16S rDNA analysis. This study is the first complex
analysis of estuarine microbiota based on isolation of
total DNA from a biome that was further subjected to
sequencing. DNA was extracted from water samples and
sequenced on the Illumina Miseq platform using primers
to the V4 variable region of the 16S rRNA gene. Computer
analysis of the obtained raw sequences was done
with QIIME (Quantitative Insights Into Microbial Ecology)
software. As the outcome, 57970 nucleotide sequences
were retrieved. Bioinformatic analysis of bacterial community
in the studied samples demonstrated a high taxonomic
diversity of Prokaryotes at above genus level. It
was shown that majority of 16S rDNA bacterial sequences
detected in the estuarine samples belonged to phyla
Cyanobacteria, Proteobacteria, Bacteroidetes, Actinobacteria,
Verrucomicrobia, Planctomycetes. The Khadhzibey
estuary was dominated by the Proteobacteria phylum,
while Dniester and Sukhyi estuaries were characterized
by dominance of Cyanobacteria. The differences in bacterial
populations between the Khadzhibey, Dniester
and Sukhyi estuaries were demonstrated through the
Beta-diversity analysis. It showed that the Khadzhibey
estuary’s microbial community significantly varies from
the Sukhyi and Dniester estuaries. The majority of identified
bacterial species is known as typical inhabitants
of marine environments, however, for 2.5% of microbial
population members in the studied estuaries no relatives
were determined.
Опис
Ключові слова
prokaryotic diversity, Black Sea estuaries, 16S rDNA analysis, metagenomic analysis
Бібліографічний опис
Acta Biochimica Polonica