Здатність диких штамів Agrobacterium spp. до адгезії
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Дата
2019
Автори
Науковий керівник
Укладач
Редактор
Назва журналу
ISSN
E-ISSN
Назва тому
Видавець
Одеський національний університет імені І. І. Мечникова
Анотація
Здатність фітопатогена до адгезії визначає успішність інфікування ним рослин-
господарів (Burr et al., 1999). Вивчення адгезивних властивостей патогенних штамів, виділених
у певному агроценозі, дозволить оцінити вірогідність виникнення епіфітотії та шлях захисту
рослин від цього захворювання. Бактеріальний рак винограду, що спричиняється Agrobacterium
vitis і A. tumefaciens (за новою номенклатурою згідно Young et al., 2001 – Rhizobium vitis і
R. radiobacter), є поширеним захворюванням на півдні України (Ліманська та ін., 2008).
The study of bacteria of the genus Agrobacterium, both collection strains, and wild strains isolated from vineyards, was conducted. Isolated from grape, which germinate in the Odessa region, Agrobacterium spp. strains showed different adhesion activity to different substrates (potato and carrot). Adhesive activity varied from (8,7 ± 1,9) × 106 CFU/ml to (2,4 ± 0,2) × 108 CFU/ml. The most active adhesion to potato tissue was demonstrated by such strains of Agrobacterium spp. as A4, A17, A18, A21; to carrot tissues – by Agrobacterium spp. A17. Investigated strains were comparing with A. tumefacies C58 and pjZ strains. Comparing to carrot cultures, adhesion to potato substrate was much more active. On potato tissues, most wild strains attached better than collectible ones. Thus, the number of adherent bacteria of wild strains of the genus Agrobacterium exceeded the number of adhered bacteria of the A. tumefaciens C58 control strain from 7 to 23.5 times, and for the museum strain A. tumefaciens pJZ - by 6.7 - 86.4 %. Experiment with carrot substrate shows less results, however, almost all strains showed largest adhesive ability, compared to control strains. In this case of carrot tissues, on the contrary, the adhesive activity of most wild strains of the genus Agrobacterium was less than the adhesive activity of the museum strain A. tumefaciens pJZ by 6.8 - 42.6 %; however, almost all strains, except strain Agrobacterium sp. A21, exceeded the adhesive activity of the collection strain A. tumefaciens C58. The shift began from 32.1 % to twice the excess. Author express gratitude to Scientific Supervisor Dr. Nataliia Limanska for guiding of the experiments and correcting the manuscript, Iryna Marynova – for provided wild strains of agrobacteria, which were isolated from grapevines, Dr. Clay Fuqua (USA) and Dr. Igor Golovlyov (Sweden) for kindly provided strain A. tumefaciens pJZ, and Iryna Marynova – for the transport of strain.
The study of bacteria of the genus Agrobacterium, both collection strains, and wild strains isolated from vineyards, was conducted. Isolated from grape, which germinate in the Odessa region, Agrobacterium spp. strains showed different adhesion activity to different substrates (potato and carrot). Adhesive activity varied from (8,7 ± 1,9) × 106 CFU/ml to (2,4 ± 0,2) × 108 CFU/ml. The most active adhesion to potato tissue was demonstrated by such strains of Agrobacterium spp. as A4, A17, A18, A21; to carrot tissues – by Agrobacterium spp. A17. Investigated strains were comparing with A. tumefacies C58 and pjZ strains. Comparing to carrot cultures, adhesion to potato substrate was much more active. On potato tissues, most wild strains attached better than collectible ones. Thus, the number of adherent bacteria of wild strains of the genus Agrobacterium exceeded the number of adhered bacteria of the A. tumefaciens C58 control strain from 7 to 23.5 times, and for the museum strain A. tumefaciens pJZ - by 6.7 - 86.4 %. Experiment with carrot substrate shows less results, however, almost all strains showed largest adhesive ability, compared to control strains. In this case of carrot tissues, on the contrary, the adhesive activity of most wild strains of the genus Agrobacterium was less than the adhesive activity of the museum strain A. tumefaciens pJZ by 6.8 - 42.6 %; however, almost all strains, except strain Agrobacterium sp. A21, exceeded the adhesive activity of the collection strain A. tumefaciens C58. The shift began from 32.1 % to twice the excess. Author express gratitude to Scientific Supervisor Dr. Nataliia Limanska for guiding of the experiments and correcting the manuscript, Iryna Marynova – for provided wild strains of agrobacteria, which were isolated from grapevines, Dr. Clay Fuqua (USA) and Dr. Igor Golovlyov (Sweden) for kindly provided strain A. tumefaciens pJZ, and Iryna Marynova – for the transport of strain.
Опис
Ключові слова
Agrobacterium, адгезивна активність, ізоляти, музейні штами
Бібліографічний опис
Збірка матеріалів наукового товариства студентів, аспірантів і молодих вчених. – Одеса: Репозитарій наукової бібліотеки ОНУ імені І.І. Мечникова, 2019.