Application of pcr markers for detecting 1BL.1RS wheat-rye chromosome translocations and (1 В) 1R substitutions

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Одеський національний університет імені І. І. Мечникова
Aims. Molecular-genetic and cytological analyses were carried out to detect the alien genes in original introgression stocks and to investigate their inheritance. Methods. Rye (Xremsl303, SRIR003) and wheat (XgwmJ8-1 Bs, Xgwm550-1B$, XgwmI40-1BL Xgwml53-IBL, Xgwm357-IAL, Taglut-1As) microsatellites and secalin-specific STS-marker (ω-sec-P3+ ω-.sec-P4) have been applied. Results. The (IB)1R wheat-rye chromosome substitution and 1BL.IRS translocation have been identified. The pairing between short arms of the 1 ВL .1 Rs translocation and of bread wheat chromosome 1В was observed with very low frequency (in 0.3% PMCs). Conclusions. The stocks have (1B)1R wheat-rye chromosome substitution or 1BL. 1Rs translocation. The translocation was contributed by the collection strains, derived from wheat cv. Avrora and originated from Petkus rye. The intact rye chromosome IR for the substitution was contributed by triticale (8x) cv. AD825 and originated from rye Voronezhskaya SHI. The substitution stocks were susceptible to leaf and stem rusts because of another origination of the 1R chromosome. Three major linked genes determining hairiness of the leaf upper surface (HLup). lower surface (HLlow) and leaf margin (Hlm) were revealed. The genes were contributed by a synthetic (T. timopheevii/Ae. tauschii) and were non-allelic to Hll gene.
Ключові слова
triticum aestivum, (1 В) IR substitution, hairy leaf, PCR-markers, IBl, IRs translocation
Бібліографічний опис
Фактори експериментальної еволюції організмів = Факторы экспериментальной эволюции организмов = Faktors in experimental evolution of organisms : зб. наук. пр. / НАН Украины, Ин-т молекулярной биологии и генетики, Укр. т-во генетиків і селекціонерів ім. М.І. Вавилова ; гол. ред. В. А. Кунах . – Київ : Логос, 2014