Перегляд за Автор "Yakimova, R."

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  • Ескіз
    Документ
    Optical Biosensors Based on ZnO Nanostructures: Advantages and Perspectives. A Review
    (2016-06) Tereshchenko, Alla V.; Bechelany, Mikhael; Viter, Roman; Khranovskyy, Volodymyr; Smyntyna, Valentyn A.; Starodub, M. F.; Yakimova, R.; Терещенко, Алла Владимировна; Терещенко, Алла Володимирівна
    This review article highlights the application of beneficial physico-chemical properties of ZnO nanostructures for the detection of wide range of biological compounds. As the medical diagnostics require accurate, fast and inexpensive biosensors, the advantages inherent optical methods of detection are considered. The crucial points of the immobilization process, responsible for biosensor performance (biomolecule adsorption, surface properties, surface defects role, surface functionalization etc.) along with the interaction mechanism between biomolecules and ZnO are disclosed. The latest achievements in surface Plasmon resonance (SPR), surface enhanced Raman spectroscopy (SERS) and photoluminescence based biosensors along with novel trends in the development of ZnO biosensor platform are presented.
  • Ескіз
    Документ
    ZnO thin films as a platform for optical immunosensors devoted for determination of GVA-antigen
    (2016) Tereshchenko, Alla V.; Fedorenko, V.; Smyntyna, Valentyn A.; Konup, Ihor P.; Konup, A.; Eriksson, M.; Yakimova, R.; Ramanavicius, A.; Balme, S.; Bechelany, Mikhael; Конуп, Ігор Петрович; Конуп, Игорь Петрович; Терещенко, Алла Володимирівна; Терещенко, Алла Владимировна
    Optical immunosensor for the determination of Grapevine virus A-type (GVA) proteins (GVAantigens) has been developed [1]. The immunosensor was based on thin films of ZnO (100 nm thickness) formed by atomic layer deposition (ALD). The ZnO-based films have demonstrated good surface-structural properties for the absorption of antibodies (anti-GVA) against GVAantigens in order to form a biosensitive layer. The absorption of anti-GVA antibodies resulted in new photoluminescence band appearance in the region of 400-550 nm that can be caused by the formation of some chemical bounds during the anti-GVA adsorption on ZnO surface [2]. The possibility to detect GVA-antigens without additional labels (e.g enzymes or fluorescent dyes) has been demonstrated. The GVA-antigen detection was performed by the evaluation of changes and behavior of the photoluminescence band, related to protein adsorption (Fig. 1). The sensitivity of as-formed label-free biosensor towards the GVA-antigens was determined in the range from 1 pg/ml to 10 ng/ml; in addition, the selectivity of biosensor was evaluated