ZnO thin films as a platform for optical immunosensors devoted for determination of GVA-antigen
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Дата
2016
Автори
Tereshchenko, Alla V.
Fedorenko, V.
Smyntyna, Valentyn A.
Konup, Ihor P.
Konup, A.
Eriksson, M.
Yakimova, R.
Ramanavicius, A.
Balme, S.
Bechelany, Mikhael
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Анотація
Optical immunosensor for the determination of Grapevine virus A-type (GVA) proteins (GVAantigens) has been developed [1]. The immunosensor was based on thin films of ZnO (100 nm thickness) formed by atomic layer deposition (ALD). The ZnO-based films have demonstrated good surface-structural properties for the absorption of antibodies (anti-GVA) against GVAantigens in order to form a biosensitive layer. The absorption of anti-GVA antibodies resulted in
new photoluminescence band appearance in the region of 400-550 nm that can be caused by the formation of some chemical bounds during the anti-GVA adsorption on ZnO surface [2]. The possibility to detect GVA-antigens without additional labels (e.g enzymes or fluorescent dyes) has
been demonstrated. The GVA-antigen detection was performed by the evaluation of changes and behavior of the photoluminescence band, related to protein adsorption (Fig. 1). The sensitivity of
as-formed label-free biosensor towards the GVA-antigens was determined in the range from 1 pg/ml to 10 ng/ml; in addition, the selectivity of biosensor was evaluated
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Ключові слова
optical immunosensor, Grapevine virus A-type (GVA), thin films of ZnO
Бібліографічний опис
Advances in Bioelectrochemistry and Nanomaterials [Электронный ресурс] : book of abstracts of the 9th Nanoconference (20th-22nd October 2016) / Vilnius University (VU) . – Электрон. кн. – Vilnius, 2016