Перегляд за Автор "Blagodarova, O. M."

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  • Ескіз
    Документ
    Association between allelic variants of Gli-B1 locus and polymorphism of microsatellite locus Taglgap in bread wheat varieties
    (Одеський національний університет імені І. І. Мечникова, 2021) Popovych, Yuliia A.; Blagodarova, O. M.; Chebotar, Sabina V.; Чеботар, Сабіна Віталіївна; Попович, Юлія Андріївна
    Gliadins are one of two main wheat storage proteins that form gluten complex and thus determine flour breadmaking properties. Therefore, gliadin genes are an important trait/marker for breeding. Gliadin genes are localized at six main loci on the 1-st and 6-th chromosomes of each subgenome and eight minor. Two classifications of gliadins are developed on the basis of acid PAGE method: by Sozinov and Popepelya [1996] (often used in Ukraine) and by Metakovsky et al. [1995] (international). Every allelic variant in this case is a complex of linked polypeptide genes inherited as one simple Mendelian trait. Due to the complexity of APAGE method, it is possible to detect some allelic variants by PCR method during selection and breeding process. The aim of the study was to explore the polymorphism of microsatellite locus Taglgap and to analyze its association with polymorphism of allelic variants of gliadins.
  • Ескіз
    Документ
    Bioinformatic analysis of nucleotide sequences of Gli‑1 loci of Triticum aestivum l.
    (Одеський національний університет імені І. І. Мечникова, 2022) Popovych, Yuliia A.; Blagodarova, O. M.; Chebotar, Sabina V.; Попович, Юлія Андріївна; Благодарова, O. M.; Чеботар, Сабіна Віталіївна
    The wheat seed storage protein, gliadin, has immense and well studied polymorphism based on the multiple allelism at each of the six main Gli loci. Therefore, gliadin alleles provide a set of suitable genetic markers for the identification and comparison of wheat genotypes.
  • Ескіз
    Документ
    Preference of molecular markers for detecting allelic variants of gliadins
    (Одеський національний університет імені І. І. Мечникова, 2023) Popovych, Yuliia A.; Blagodarova, O. M.; Chebotar, Sabina V.; Попович, Юлія Андріївна; Чеботар, Сабіна Віталіївна
    The main method of studying allelic variants of gliadins is electrophoresis in acidic PAGE, which actually shows the phenotype determined by the set of genes of the gliadin-encoding locus. This method is difficult to use, because peptides encoded by all gliadin-encoding loci are present on one lane of the gel and may overlap. Therefore, there is a need for molecular markers that would allow the determination of allelic variants of gliadins using PCR. This will help to use gliadins for marker-asissted selection, to identify allelic variants of gliadins even before obtaining grain suitable for electrophoresis of storage proteins. In this regard, the aim of study was to analyze polymorphism of Gli‑1 loci using allele-specific molecular markers and microsatellites and find suitable molecular markers for detecting allelic variants of gliadins by PCR method.