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Документ Antagonistic activity of Lactobacillus plantarum against crown gall agent(2013) Limanska, Natalia V.; Korotaeva, Nadiya V.; Biscola, Vanessa; Ivanytsia, Tetіana V.; Basiul, Olena V.; Choiset, Yvan; Merlich, Andrii H.; Kondratiuk, Tetiana V.; Yamborko, Hanna V.; Nazarenko, Hanna M.; Chobert, Jean-Marc; Ivanytsia, Volodymyr O.; Haertle, Thomas; Ямборко, Анна Валентиновна; Ямборко, Ганна Валентинівна; Лиманская, Наталья Викторовна; Ліманська, Наталія Вікторівна; Коротаева, Надежда Владимировна; Коротаєва, Надія Володимирівна; Бискола, Ванесса; Біскола, Ванесса; Иваница, Татьяна Владимировна; Іваниця, Тетяна Володимирівна; Басюл, Олена Владленівна; Басюл, Елена Владленовна; Мерлич, Андрей Геннадиевич; Мерліч, Андрій Геннадійович; Кондратюк, Тетяна В.; Кондратюк, Татьяна В.; Назаренко, Ганна Миколаївна; Назаренко, Анна Николаевна; Іваниця, Володимир Олексійович; Иваница, Владимир АлексеевичThe aim of this work was to study the antagonistic effect of Lactobacillus planlarum on phytopathogenic agrobacteria. Lactobacilli isolated from various plant sources have been identified by microbiological and molecular biological methods and a LAB collection has been formed. Physiological, biochemical, molecular biological and technological properties of the isolated bacteria were characterized.Документ Study of the Potential Application of Lactic Acid Bacteria in the Control of Infection Caused by Agrobacterium tumefaciens(2015) Limanska, Natalia V.; Korotaeva, Nadiya V.; Biscola, Vanessa; Ivanytsia, Tetіana V. ; Merlich, Andrii H.; Franco BDGM; Chobert, JM; Ivanytsia, Volodymyr O.; Haertlé, T.; Іваниця, Володимир Олексійович; Мерліч, Андрій Геннадійович; Мерлич, Андрей Геннадиевич; Іваниця, Тетяна ВолодимирівнаInhibition of crown gall on test plants in case of co-inoculation with lactic acid bacteria (LAB) has been investigated. From nine LAB strains tested, eight reduced amount of galled carrot explants by 36.4-87.7% and decreased the intensity of disease manifestation. The antagonistic activity against Agrobacterium tumefaciens, in vitro, was due to the low pH of organic acids produced by LAB. However, in the same pH, different LAB cultures displayed various levels of inhibition in vivo. Lactobacillus plantarum ONU 12 with the best results in tumor inhibition on carrots, showed high antagonistic activity on surfaces of kalanchoe and grapevines. Depending on the method of inoculation, the culture of L. plantarum ONU 12 could protect from 72.7% to 100% of wounded kalanchoe tissues. Evaluation of number of surviving cuttings and amount of buds that grew indicated that co-inoculation with agrobacteria and LAB removed completely the negative influence of phytopathogen on grapevines and reduced the number of infected cuttings by approximately 80%. One-hour treatment with L. plantarum ONU 12 helped to decrease the number of infected plants by approximately 68%. The studied strain L. plantarum ONU 12 can be proposed for further evaluation of possibility of practical use in plant protection.