Перегляд за Автор "Tkachenko, A."

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  • Ескіз
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    Biological properties of bacteria Bacillus subtilis ONU551 and Aeromonas ichthiosmia ONU552 – phenol destructors
    (Одеський національний університет імені І. І. Мечникова, 2020) Tkachenko, A.; Horshkova, Olena H.; Горшкова, Олена Георгіївна; Горшкова, Елена Георгиевна; Radysh, N.; Pihteeva, A.; Smazchuk, O.; Lebedeva, E. V.; Poschelyuk, A. A.
    The biological properties of bacteria of Bacillus subtilis ONU551 and Aeromonas ichthiosmia ONU552 are studied - destructions of phenol. The stamm of Bacillus of subtilis of ONU551 is presented by gram-positive sticks that form субтермінально ендоспори is located. A stamm of Aeromonas ichthiomia ONU552 is gram-negative direct sticks. The features of fat-acid composition of stamms of Aeromonas ichthiomia ONU552 ONU552, B. subtilis ONU551 of destructions - to the phenol, that distinguish them from other bacteria - in cellular lipids each of stamms presence of fat acids : 16: 1 w7c alcohol, 17: 0 іso, 17: 0 anteiso.
  • Ескіз
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    Efficiency of purification of water from cyclic aromatic xenobiotics with strains P. fluorescens ONU328 and P. maltophilia ONU329
    (Одеський національний університет імені І. І. Мечникова, 2018) Horshkova, Olena H.; Voliuvach, Olha V.; Tkachenko, A.; Pihteeva, A.; Smazchuk, O.; Haidarzhi, A.; Волювач, Ольга Вячеславовна; Волювач, Ольга В'ячеславівна; Горшкова, Олена Георгіївна; Горшкова, Елена Георгиевна
    Abstract. It has been experimentally established that non-pathogenic strains of bacteria of the genus Pseudomonas, identified by the fatty acid composition of their cellular lipids as P. fluorescens ONU328, P. maltophilia ONU329 (isolated from the marine environment), have oxidizing ability for cyclic aromatic xenobiotics (phenol, N-cetylpyridinium bromide). The high phenol-oxidizing ability of the investigated strains of microorganisms is proved. When water of bacterial cells in the amount of 7.5 x 105 CFU/ ml is introduced into phenol contaminated (at a concentration of 300 mg / l), the degree of water purification from phenol is 100% on day 18 when using as a biodestructor strain P. maltophilia ONU329 and 22 days - when using strain P. fluorescens ONU328. It has been experimentally confirmed that when the free cells of bacteria of P. fluorescens ONU328 or P. maltophilia ONU329 strains in the amount of 5.5x104 CFU/ ml are introduced once into the contaminated water, the purification rate of water from N-cetylpyridinium bromide at a concentration of 20 mg / l reaches 50.7% for the 4th day and 55.7% for the 6th day, respectively. With the repeated introduction of fresh portions of microorganism-destructors or use of higher starting doses (5.5x10 CFU/ ml), the water purification rate from N-CPB is 98.0-98.5%.
  • Ескіз
    Документ
    Method of eliminating oil film pollution on the water area
    (2015) Horshkova, Olena H.; Voliuvach, Olha V.; Piddubnaya, S.; Pototskaya, A.; Kovtalo, M. S.; Tkachenko, A.; Волювач, Ольга В'ячеславівна; Волювач, Ольга Вячеславовна; Горшкова, Елена Георгиевна; Горшкова, Олена Георгіївна
    The method of using the biopreparation – a sorbent and destructor of oil hydrocarbons which block oil pollution of the aquatic environment in the shortest possible time prevents spreading and eliminates it with minimum ecological loss.
  • Ескіз
    Документ
    Microbiological method of purification of water from phenol association of non-pathogenic bacteria straps of the genus Pseudomonas
    (Odesa I. I. Mechnikov National University, 2018) Horshkova, Olena H.; Voliuvach, Olha V.; Samofalov, Mykhailo O.; Tkachenko, A.; Smazchuk O.; Pihteeva, A.; Gaydarzhi, A.; Горшкова, Олена Георгіївна; Волювач, Ольга В'ячеславівна; Самофалов, Михайло Олександрович
    A microbiological method for purifying water from phenol has been developed, which consists in using phenol-bacterial association of strains of non-pathogenic bacteria of the genus Pseudomonas: P. cepacia ONU-327 and P. fluorescens ONU- 328, taken in a volume ratio of 1: 1. When using a biopreparation on the basis of the association of the bacterial strains under study, the oxidation of phenol is accelerated 2.2 times as compared to the treatment of phenol-containing water with monocultures. Deep purification of water from phenol with a concentration of 300 mg/l occurs within 10 days with a single injection of bacterial cells in an amount of 7.5x105 CFU/ml, pH 7, a temperature of 28-30 °C. It has been established that the bacterial association of strains of P. cepacia ONU-327 and P. fluorescens ONU-328 is capable not only of effective destruction of phenol, but also possesses a high sorption-accumulating potential for ions of heavy metals [Pb (II), Zn (II) , Cu (II), Cr (V)], which reveals the prospects of its use in the biopreparation for the purification of multicomponent pharmacies, with the predominant content of phenolic contaminants.
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    The use of wine yeast in the production of red wines in biotechnology
    (Одеський національний університет імені І. І. Мечникова, 2018) Haidarzhi, A.; Tkachenko, A.; Smazchuk, O.; Pihteeva, A.
    The experiments conducted indicate that yeast Saccharomyces vini the studied race siha aktivhefe 4 refer to glucosophilic yeasts of the genus Saccharomces, fermenting the most significant fraction of sugar in grape juice. These yeasts end up fermentation, and many of them are involved in the formation of the aroma of young red wines. Yeast assimilate and ferment glucose, galactose, sucrose, maltose and raffinose (by 1/3), do not absorb and do not ferment dextrins, lactose, inulin, xylose, arabinose. Assimilate ethyl alcohol and glycerin, do not assimilate mannitol, dulcite, sorbitol. From organic acids, they absorb acetic and lactic acid, they do not absorb amber, apple, wine and lemon. In winemaking, yeast can play a negative role, causing turbidity of the finished wines.