Перегляд за Автор "Nidialkova, N. A."

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  • Ескіз
    Документ
    Complexes of biscitratogermanates and biscitratostanates with metals are modifiers of bacillus thuringiensis var. іsraelensis peptidases and penicillium canescens, cladosporium cladosporioides and aspergillus niger α- galactosidases activities
    (2016) Varbanets, L. D.; Nidialkova, N. A.; Borzova, N. V.; Seifullina, Inna Y.; Martsynko, Olena E.; Chebanenko, Olena Anatoliivna; Варбанець, Л. Д.; Нідялкова, Н. А.; Борзова, Н. В.; Сейфулліна, Інна Йосипівна; Марцинко, Олена Едуардівна; Чебаненко, Олена Анатоліївна; Варбанец, Л. Д.; Нидялкова, Н. А.; Борзова, Н. В.; Сейфуллина, Инна Иосифовна; Марцинко, Елена Эдуардовна; Чебаненко, Елена Анатольевна
    The aim of the research was to study the effect of a number of germanium and stanum coordination compounds (compounds 1–8) as modifiers of peptidases and α-galactosidases activity. The coordination compounds of the same type of structure based on chelating ligand that is biologically activities citric acid were investigated as enzymes effectors. Two types of complexes: 1) [M(H2O)6][Ge(НCitr)2]4H2O (M = Mg(1), Mn(2), Co(3), Ni(4), Zn(5)), containing biscitrate-germanate anion ([Ge(НCitr)2]2–), and 2) [M(H2O)6][Sn(НCitr)2]4H2O (M = Mg(6), Co(7), Ni(8)), containing biscitrate-stanate anion and various hexaaquacations ([M(H2O)6]2+, М= Mg, Mn, Co, Ni, Zn) were studied. It is shown that the compound 6, which is biscitrate-stanate complex containing magnesium ions as metal, can be used for the stimulation of B. thuringiensis var. israelensis IMV B-7465 peptidase 1 and peptidase 2 collagenase activity by 20–25%. Compounds 1 (biscitrate-germanate complex containing magnesium ions as metal) and 7 (biscitrate-stanate complex containing cobalt ions as metal), and also compound 6 in a concentration of 0.001% are able to increase elastolytic activity of peptidase 1 by 55–58%. However, compound 7 has shown the greatest activating effect. It increased the elastolytic activity of peptidase 2 by 100–140% (at both tested concentrations). This goes to prove that the compound 7 can be used henceforth as effector of peptidase 2 elastolytic activity. While investigating the effect of the considered coordination compounds on the Penicillium canescens, Cladosporium cladosporioides and Aspergillus niger -galactosidase activity, it has been found that, when using a number of complexes (1–2 and 4–8), there is a slight increase (by 12–20%) of P. canescens enzyme activity, and the maximal effect (~20%, concentration 0.01%) was provided by complex 6.
  • Ескіз
    Документ
    He effect of Stanum (IV) and Germanіum (IV) coordination compounds on Bacillus thuringiensis var. israelensis ІМV В-7465 peptidases activity
    (2015) Nidialkova, N. A.; Varbanets, L. D.; Seifullina, Inna Y.; Shmatkova, Natalia Volodymyrivna; Нідялкова, Н. А.; Варбанець, Л. Д.; Сейфулліна, Інна Йосипівна; Шматкова, Наталя Володимирівна; Нидялкова, Н. А.; Варбанец, Л. Д.; Сейфуллина, Инна Иосифовна; Шматкова, Наталья Владимировна
    The purpose of the research was to investigate the influence of Stannum (IV) and Germanium (IV) coordination compounds on peptidases 1 and 2 of Bacillus thuringiensis var. israelensis ІМV В-7465 activity. The study of coordination compounds effect on peptidase activity was carried out by complexes with the enzymes incubation and residual activity in relation to collagen, elastin and fibrin determination. It was revealed the regularities in the influence of different structure complex compounds on peptidases of B. thuringiensis var. israelensis ІМV В-7465 activity. Stannum (IV) complexes with salicyloylhydrazones of aromatic aldehydes increase collagenase and elastase activities. Substituents replacement in aldehyde fragment of Stannum (IV) complexes with isonicotinoylhydrazones of aromatic aldehydes by the less polar ones contributed to increase of elastase activity of both enzymes while an absence of substituents enables to increase peptidase 2 fibrinolytic activity. The different metal complexes of Germanium (IV) with isonicotinoylhydrazone of salicylic aldehyde, which contain Zn and Co, increased collagenase activity of the peptidase 1 as well as elastase and fibrinolytic activities of the peptidase 2. In general, all tested complexes may be considered as peptidase effectors of B. thuringiensis var. israelensis ІМV В-7465. The difference of complexes effect on activities of the both enzymes is due to the characteristics of the structure of coordination compounds