Перегляд за Автор "Nesterkina, Maria V."

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    Документ
    Anti-inflammatory and analgesic activity of ointment based on dense ginger extract (Zingiber officinale)
    (2019) Kravchenko, Iryna A.; Eberle, Lidiia V.; Nesterkina, Maria V.; Kobernik, Alona O.; Кравченко, Ірина Анатоліївна; Еберле, Лідія Вікторівна; Нестеркіна, Марія Володимирівна; Кобернік, Альона Олександрівна; Кравченко, Ирина Анатольевна; Нестеркина, Мария Владимировна; Коберник, Алёна Александровна
    Introduction: Zingiber officinale (Zingiberaceae family) is traditionally used in alternative medicine to reduce pain from rheumatoid arthritis and osteoarthritis. Ginger is also often applied for stomach and chest pain, toothaches and as anti-inflammatory agent. The aim of this study is to investigate analgesic and anti-inflammatory activities of Z. officinale dense extract after its transdermal delivery using allyl isothiocyanate (AITC) induced model with further discussion of possible action mechanism of ginger phytoconstituents. Methods: Inflammation was induced by subplantar injection to the plantar fasciitis (aponeurosis) of the hind limb of rats using 30 μL AITC solution (100 μg/limb) in 1,2-propyleneglycol. The dynamics of changes of inflammatory process was evaluated before addition of the inflammation inducer and after 1, 2, 3, 4, 6 and 24 hours of its injection for measuring the volume and the thickness of affected limb. Analgesic activity of ointments with ginger extract was examined using the model of AITC-induced pain. Results: The most effective inhibition of the development of inflammation process was 0.025% ointment with ginger extract, and the highest anti-nociceptive effect was observed at the application of 0.05% ointment 10 minutes before pain inducer agent. Conclusion: Zingiber officinale dense extract was revealed to possess significant antinociceptive and anti-inflammatory actions after its transdermal delivery. Since the pharmacological effects of ginger extract have been investigated on AITC-induced model, we may suggest the vital role of phytoconstituents binding to TRPA1 and TRPV1 ion channels as possible mechanism of action.
  • Ескіз
    Документ
    Pharmacotherapy of inflammatory process by ginger extract (Zingiber officinale) ointment
    (2019) Kravchenko, Iryna A.; Eberle, Lidiia V.; Nesterkina, Maria V.; Kobernik, Alona O.; Кравченко, Ірина Анатоліївна; Еберле, Лідія Вікторівна; Нестеркіна, Марія Володимирівна; Кобернік, Альона Олександрівна; Кравченко, Ирина Анатольевна; Нестеркина, Мария Владимировна; Коберник, Алёна Александровна
    Introduction: The anti-inflammatory activity of the ointment based on ginger extract was investigated on carrageenan-induced inflammation. Methods: Anti-inflammatory activity was investigated on the carrageenan inflammation model in rat. The animals were divided into 5 groups: first group– control (without treatment); second group– animals received preventive treatment of ointment with ginger extract two days before experiment and with subsequent treatment after administration of phlogogen; 3rd group– animals received applications of 5% ibuprofen 2 days before experiment and with subsequent treatment after induction of inflammation; fourth group – animals were treated with ginger extract ointment after one day of the phlogogen administration; fifth group – animals were treated with reference drug after one day of the phlogogen introduction. The biochemical parameters (concentration of sialic acids, seromucoids, contents of total proteins and protein fractions) of the blood of experimental animals were investigated. Results: After local application of the ointment from the ginger extract, under the conditions of its application after phlogogen inoculation, the effect was the same as anti-inflammatory effect of the ibuprofen which was used as reference drug. The preventive ointment application provided faster and more intense reduction of the inflammatory focus with returning to the background indicators on the eighth day of the experiment, and for biochemical parameters on the sixth day of therapy. Conclusion: The ointment from ginger extract shows anti-inflammatory activity by inhibiting inflammation caused by carrageenan.