Перегляд за Автор "Mashkova, A. K."

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    Antagonistic activity of actinobacteria isolated from sponges Haliclona spp. Odesa Bay
    (Одеський національний університет імені І. І. Мечникова, 2024) Strashnova, Iryna V.; Mashkova, A. K.; Lisiutin, G. V.; Ivanytsia, Tetіana V.; Страшнова, Ірина Валентинівна; Машкова, А. К.; Лісютін, Г. В.; Іваниця, Тетяна Володимирівна
    The marine ecosystem is a promising source for discovering the producers of totally new bioactive compounds. Among the significant biodiversity, actinobacteria also are known by huge antagonistic potential. Aim. To study the antagonistic activity of actinobacteria isolated from marine sponges Haliclona spp. against the strains of indicator prokaryotic and eukaryotic microorganisms. Methods. Antagonistic activity of actinobacteria isolated from marine sponges Haliclona spp. against Staphylococcus aureus АТСС 25923, Micrococcus luteus АТСС 4698, Enterococcus faecalis АТСС 29212, Bacillus subtilis АТСС 6633, Kocuria rhizophila DSM 348, Escherichia coli АТСС 25922, Proteus vulgaris АТСС 6896, Salmonella enterica NCTC 6017, Klebsiella pneumoniae АТСС 10031, Pseudomonas aeruginosa АТСС 27853, Pseudomonas putida KT 2440 and Candida albicans ATCC 18804 were determined in vitro by the block method. Results. Thirteen from fourteen strains of actinobacteria inhibit the growth of at least one strain of the indicator microorganism. The most active strains of actinobacteria were Hal 2 and Hal 14, that significantly inhibit the growth of fifth strains of indicator microorganisms, the spectrum of which were differed. Strain Hal 2 has ability to inhibit the growth of S. aureus ATCC 25923, M. luteus ATCC 4698, E. faecalis ATCC 29212, K. rhizophila DSM 348, P. aeruginosa ATCC 27853 and C. albicans ATCC 18804, strain Hal 14 inhibit all Gram-positive bacteria, including B. subtilis ATCC 6633. K. rhizophila DSM 348, M. luteus ATCC 4698 and P. aeruginosa ATCC 27853 shown the impressive sensitivity to the studied strains of actinobacteria. The growth of P. aeruginosa was inhibited by ten strains of actinobacteria. Four strains of actinobacteria (Hal 2, Hal 4, Hal 5 and Hal 6) inhibit the growth of C. albicans ATCC 18804 with different intensity. All the indicators of the family Enterobacteriaceae and P. putida KT 2440 were resistant to the studied strains of actinobacteria. Conclusions. Strains of actinobacteria isolated from marine sponges Haliclona spp. inhibit the growth of indicator strains of prokaryotic and eukaryotic microorganisms. S. aureus АТСС 25923, M. luteus АТСС 4698, E. faecalis АТСС 29212, K. rhizophila DSM 348, P. aeruginosa ATCC 27853 and C. albicans ATCC 18804 are the most sensitive to the antagonistic action of actinobacteria. Strains of actinobacteria Hal 2, Hal 4 and Hal 14, which suppressed the ability to grow of the large quantity of indicator microorganisms with significant zones of inhibition of the growth, were chosen for further studies of the spectrum and properties of their secondary metabolites.