Перегляд за Автор "Kolomiets, O. G."

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  • Ескіз
    Документ
    Biodestruction of phenol by non-pathogenic bacteria of the genus Pseudomonas
    (Odessa I. I. Mechnikova National University, 2017) Horshkova, Olena H.; Voliuvach, Olha V.; Bebyk, V. V.; Kolomiets, O. G.; Lagovskaya, L. S.; Khadzhy, V. D.; Волювач, Ольга Вячеславовна; Волювач, Ольга В'ячеславівна; Горшкова, Олена Георгіївна; Горшкова, Елена Георгиевна
    It has been experimentally established that two strains of bacteria genus Pseudomonas, isolated from the marine environment and identified by the fatty acid composition of their cellular lipids as P. fluorescens ONU328, P. maltophilia ONU329, are not pathogenic and have a high phenol-oxidizing ability. Bacteria are cultured for two days at a temperature of 30 °C on MPA, further bacterial cells are suspended in the mineral medium M-9, and in the optimum amount is introduced into the contaminated water containing up to 300 mg/l phenol. It was established that the strain of P. maltophilia possessed a greater phenol-oxidizing activity than the strain P. fluorescens. When water was treated with strain P. maltophilia with a culture concentration of 3.5×104 CFU/ml, deep purification from phenol was observed on 34 day, and with an increase in the culture concentration to 7.5×105 CFU /ml on 18 day, t=30 °C.
  • Ескіз
    Документ
    Biomarker values of nonpathenous microorganisms with polyfunctional biotechnological properties
    (Odessa I. I. Mechnikova National University, 2017) Horshkova, Olena H.; Voliuvach, Olha V.; Kolomiets, O. G.; Bebyk, V. V.; Khadzhy, V. D.; Lagovskaya, L. S.; Волювач, Ольга В'ячеславівна; Волювач, Ольга Вячеславовна; Горшкова, Елена Георгиевна; Горшкова, Олена Георгіївна
    Isolated from the oil-contaminated soil of Zmeiniy іsland and the marine environment are four non-pathogenic biochemically active strains of microorganisms identified by the fatty acid composition of their cell lipids as Microbacterium barkeri OZ-3, Bacillus megaterium OZ-5 (isolated from soil), Pseudomonas fluorescens ONU-328, Pseudomonas maltophilia ONU-329 (separated from the marine environment). The strains possess polyfunctional biotechnological properties: sorptionaccumulating ability relative to heavy metal ions, oxidizing activity with respect to petroleum products, anionic surfactants and phenolic compounds. Biomarker values for distinguishing the strains of microorganisms studied (Microbacterium barkeri OZ-3, Bacillus megaterium OZ-5, Pseudomonas fluorescens ONU-328, Pseudomonas maltophilia ONU-329) are proposed at the species level.