Перегляд за Автор "Danylova, Olena"

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    Methodology for accelerated monitoring and assurance of sanitary quality and food safety
    (2017) Pylypenko, Inna; Pylypenko, Liudmyla; Yamborko, Hanna V.; Danylova, Olena; Ямборко, Ганна Валентинівна; Ямборко, Анна Валентиновна
    Introduction. The microbiological criteria that ensure sanitary quality and safety of food products, methods for identifying regulated microorganisms, and the urgency of accelerated microbiological control of food safety are characterized. Materials and methods. The methodology for assessing the safety of products and the classical and accelerated methods for determining regulated microbiological indicators that identify the presence of heat-resistant pathogens of food diseases are studied. Analytical studies are based on modern literary sources and some of own results. Results and discussion. The characteristics of microbiological criteria and requirements for microbiological safety of food products were given. Analysis of modern requirements for the sanitary safety of food has shown the need for microbiological control for the presence of heat-resistant microorganisms, which are potential pathogens of foodborne diseases. Microbial species traditionally the main assessment of their health status such as Clostridium botulinum, Bacillus cereus, Clostridium perfringens, Staphylococcus aureus were given. Characteristics of the phenotypes and genotypic properties of criterial microorganisms – potential causative agents of foodborne infections and poisonings are given. The study methodology and methods of control of regulated microorganisms showed failure and inaccuracy of their phenotypic diagnosis due to the similarity of morphological and tinctorial properties within the individual groups, the variability of a number of biochemical parameters, weak antigenicity for the immunological diagnosis, the advent of new metabolic features associated with the ability to synthesize genes toxicity by microorganisms, which were traditionally considered to be nonpathogenic, labor-consuming and durable analysis. Genotypic diagnostics of microorganisms using modern molecular genetic methods and methodologies, in contrast to the phenotypic one, ensures the accuracy of identification, the ability to monitor and predict the behavior of pathogens of foodborne infections and toxic infections in products in assessing microbiological risk, allows accelerated microbiological control of food safety, taking into account their specific features of composition and properties, is a reliable method of sanitary control. Conclusions. Molecular genetic diagnosis of pathogens is a promising accelerated method for determining food safety and is relevant especially in the Ukrainian region.