Біологічний факультет
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Перегляд Біологічний факультет за Автор "Guo Jingjng,"
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Документ Development and optimisation of storage methods for mesenchymal stem cells (MSCs) and their exosomes (MSC-Exos) to preserve their therapeutic potential(Одеський національний університет імені І. І. Мечникова, 2024) Guo Jingjng,This study focuses on optimizing exosome extraction and modification methods to improve their purity, stability, and biomedical applicability. Exosomes were extracted from the supernatant of umbilical cord mesenchymal stem cells using ultracentrifugation and dialysis ultrafiltration. Comparative analysis revealed that ultracentrifugation yielded exosomes with higher purity, consistent particle size, and strong expression of specific markers (TSG101 and CD9), making it the preferred extraction method. Exosome liposomes were successfully prepared using ultrasound and thin-film dispersion methods. The ultrasound method, particularly at an optimal amplitude of 23%, achieved efficient fusion of exosomes and liposomes, as confirmed by fluorescence resonance energy transfer (FRET) technology. This method also retained the specific markers of exosomes (TSG101 and CD9). In stability tests, exosome liposomes prepared by ultrasound demonstrated superior physical and biological stability under short-term storage at 4°C, maintaining consistent particle size, PDI, and Zeta potential, while promoting better cell proliferation compared to thin-film dispersion. The degradation rates of exosome-specific markers were lower for ultrasound-prepared liposomes, supporting their enhanced functional stability. Overall, the study proposes ultracentrifugation as the optimal exosome extraction method and ultrasound (23% amplitude) as the preferred approach for preparing stable and functional exosome liposomes. These findings provide valuable insights for enhancing the biomedical applications of exosomes.