Перегляд за Автор "Finogenova, M. O."
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Документ Biofilm formation of pseudomonas aeruginosa PA01 AwspF1 strain with high c-di-GMP level(Одеський національний університет імені І. І. Мечникова, 2018) Semenets, Anastasiia S.; Finogenova, M. O.; Galkin, M. B.Bis-(3-5')-cyclic dimeric guanosine monophosphate (c-di-GMP) is in the spotlight of the scientists as the result of last achievement of microbial genomics and great interests in microbial communities [6, 8]. Depends on concentration of this regulator bacteria shifts its life-form from motile to sessile (biofilm formation) [7]. Aim was to determine swarming motility and biofilm formation abilities in P. aeruginosa PA01 AwspF1, with high c-di-GMP level.Документ Fatty acid profiles in planktonic and bi ofi lm P. aeruginosa cells with different level of c-di-GMP(2018) Halkin, Mykola B.; Semenets, Anastasiia S.; Finogenova, M. O.Pseudomonas aeruginosa is a Gram-negative pathogen responsible for a variety of opportunistic infections, including chronic airway infections in patients with cystic fibrosis (CF). During the course of chronic infection, P. aeruginosa forms matrix-encased, surface-associated communities called biofilms. Biofilms are thought to contribute to persistence in the CF airway by contributing to evasion of the host immune response and antimicrobial therapy. Biofilm communities differ from planktonic bacterial cultures not only in terms of their metabolic activity but they also display stage-specific phenotypes during development and considerable spatial heterogeneity of physiological condition. Cells alter the fatty acid composition of their lipids to maintain membrane fluidity with varying environmental conditions. In this study, fatty acid profiles of biofilms versus planktonic cells of the P. aeruginosa with different level of intracellular c-di-GMP were compared.Документ Polysaccharides production by Pseudomonas c-di-gmp mutants(2015) Finogenova, M. O.; Semenets, Anastasiia S.During the past decade, there has been a renewed interest in using Pseudomonas aeruginosa as a model system for biofilm development. Pseudomonads, and in particular Pseudomonas aeruginosa, are among the beststudied biofilm formers, due to the long history of study of these bacteria, their genetic tractability and the availability of the genome sequence (O'Toole, 2003). One of the most well studied biofilm component is matrix (extracellular polymeric substance). It is a complex of biopolymers, synthesized by microorganism in biofilm. It consists of polysaccharides, structural proteins, etc. Matrix plays an important role in biofilm architecture forming and separates biofilm from the environment. Among others matrix components are exopolysaccharides (EPS) that provides a viscous structure of matrix and allows evenly distribute unfavorable substances throughout the biofilm (antibiotics, disinfectants) (Fleming, 2010). It should be mentioned that biofilm formation is in direct proportion to the level of secondary messenger c-di-GMP biosynthesis.Документ Sensitivity determining of c-di-GMP Pseudomonas aeruginosa strains by disk diffusion method(Odesa I. I. Mechnikov National University, 2016) Lisova, O. O.; Finogenova, M. O.; Semenets, Anastasiia S.С-di-GMP controls the switch between motile and sessile lifestyles: high cellular levels of c-di-GMP promote exopolysaccharides production and surface adhesion, leading to biofilm formation; on the other hand, low c-di-GMP levels result in flagellar gene-expression and increased cellular motility (RÖmling et al., 2013). Antimicrobial therapy has played a crucial role in the treatment of infectious diseases in humans in XX century, as the use of antibiotics significantly reduced mortality from infection.