Kozoriz, A.2017-11-272017-11-272016"Molecular microbiology and biotechnology", International scientific conference": abstracts, Odessa, Ukraine, June 21-23, 2016 / Odessa I.I. Mechnikov national univ. ; Chairman: V. O. Ivanytsia [et al.] . – Odessa, 2016.https://dspace.onu.edu.ua/handle/123456789/11545In the developing countries, the lack of clean drinking water and food is a critical health problem. The World Health Organization (WHO) estimated that 1.5 million children die from diarrheal diseases every year. 88% of such diseases were caused by unsafe drinking water and inadequate sanitation of food products. Traditional microbiological cultural methods for the detection of bacterial contamination require 2 days or more. Verification of species identity requires additional morphological testing or further subcultivation in differential media. While the rapid detection of these cells it is very important to prevent the risk of foodborne diarrheal diseases. Other culture-independent methods such as those based on polymerase chain reaction (PCR) are also used for identifying bacteria in water and food samples. These DNA-targeted methods detect the presence of genes specific to bacterial species or strains and they offer good sensitivity and speed using minimal number of sample. However, PCR is unable to discriminate viable bacterial cells from dead. Such false positive results can only be verified through microbiological or other methods.enEscherichia colibacteriophage T7microorganismsArticle