Gudzenko, Tetiana V.Voliuvach, Olha V.Horshkova, Olena H.Ostapchuk, Andriy M.Ivanytsia, Volodymyr O.Гудзенко, Тетяна ВасилівнаВолювач, Ольга В'ячеславівнаГоршкова, Олена ГеоргіївнаІваниця, Володимир Олексійович2020-02-122020-02-122019The Ukrainian biochemical journalhttps://dspace.onu.edu.ua/handle/123456789/27219The aim of the study was to determine the cellular lipids fatty acid composition for identification of the Bacillus subtilis ONU 551 strain bacteria, which is a phenol destructor. Fatty acids analysis of B. subtilis ONU 551 strain was performed using an automatic system for microorganisms’ identification MIDI Sherlock (MIDI , USA ) based on gas chromatograph Agilent 7890. Chromatograms analysis showed that the fatty acid spectrum of the strain B. subtilis ONU 551 consisted predominately of branched structural isomers of saturated acids: 13-methyltetradecanoic (15:0 iso; 34.72%) and 12-methyltetradecanoic (15:0 anteiso; 33.72%) acids. The total content of the branched saturated fatty acids was 88.16% – 14:0 iso (0.52%), 15:0 iso (34.72%), 15:0 anteiso (33.72%), 16:0 iso (1.85%), 17:0 iso (7.11%), 17:0 anteiso (10.24%). The saturated fatty acids of the normal structure were also detected – 12:0 (0.36%), 14:0 (0.28%), 16:0 (1.30%). No 2- and 3-hydroxy acids and no cyclic fatty acids were detected in the fatty acid profile of B. subtilis ONU 551 strain. Unsaturated fatty acid isomers – 15:1 w5c (1.85%), 16:1 w11c (1.21%), 16:1 w7c alcohol (1.08%), 17:1 iso w10c (3.18%), Σ17:1 iso I/anteiso B (2.57%) were shown to be the distinctive biomarkers of the B. subtilis ONU 551 strain. According to the fatty acid profile analysis with MIDI Sherlock system, the studied strain was identified as Bacillus subtilis with high level of similarity index (0.563).enBacillus subtilis ONU 551fatty acids compositionsaturated acids isomersstrain identificationArticle