Перегляд за Автор "Zlatohurska, Maryna"

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  • Ескіз
    Документ
    Biofilm formation and genetic diversity of Lactobacillus plantarum strains originated from France and Ukraine
    (2019) Limanska, Natalia V.; Merlich, Andrii H.; Halkin, Mykola B.; Vasylieva, Nataliia Yu.; Choiset, Yvan; Ivanytsia, Tetіana V.; Zlatohurska, Maryna; Ivanytsia, Volodymyr O.; Chobert, Jean-Marc; Мерліч, Андрій Геннадійович; Іваниця, Володимир Олексійович; Іваниця, Тетяна Володимирівна
    Evaluation of Lactobacillus plantarum strains perspective for plant protection or fermentation of food of plant origin should include tests on abilities to attach to plant surfaces and to form biofilms. Comparing RAPD-profiles with these properties and also with geographical origin of the strains is necessary to find out the possibility of rapid selection of the strains and tracking their dissemination. Biofilm formation and RAPD-PCR profiles of 30 strains of L. plantarum isolated from grape must and pickles originated from France and Ukraine were compared. All isolates were able to attach to Lepidium sativum seedlings and form biofilms of different types. Amount of attached cells varied from (7.8 ± 0.2) x 104 to (2.1 ± 0.3) x 103 CFU/cm2. The most developed biofilms were formed on shoots, leaves, seed coat shells. On roots this ability was strain dependent: the majority of French isolates (77.0%) formed separated microcolonies without developed matrix layer. Among Ukrainian isolates, 47.1% of the strains showed the same tendency, and in 41.2% of the strains individual attached cells without formation of microcolonies were observed. The level of initial attachment of bacterial cells to roots did not coincide with the level of subsequent formation of biofilms. Gene plnA was detected in 28.1% of isolates. All plnA possessing strains except the one formed microcolonies or biofilms with developed matrix. Lactobacilli could eliminate the biofilms of Agrobacterium tumefaciens pJZ from tomato roots in 75.0 – 100% of tested samples depending on a strain and method of inoculation. No clear association between the geographical origin of the L. plantarum strains and clustering by RAPD-PCR analysis was found. Two strains with high level of biofilm formation were grouped in one subcluster.
  • Ескіз
    Документ
    Comparative study of erwiniophages 49 and 59 based on restriction analysis of their genomes
    (2015) Zlatohurska, Maryna
    Bacteriophages as vectors for obtaining recombinant DNA molecules have been widely used in genetic engineering and biotechnology. Also, it was found possible to use separate virion components for protection, delivery and introduction of medicines. Discovery of these application opportunities requires reconsideration of well known phages and isolation and investigation of new viruses of bacteria.
  • Ескіз
    Документ
    INFLUENCE OF THE BACTERIA OF GENUS PSEUDOMONAS ON TOMATO SEED SOWING CHARACTERISTICS
    (Печатный дом, 2012) Zlatohurska, Maryna
    The question of ecologically pure food production is actual in conditions of environmental degradation. The exception of the chemicals' use is necessary for obtain ecologically pure food products. The processing of tomato seeds (as a valuable food crop) by biological agents permits to reduce the use of pesticides. The purpose of the research was to study the effect of the bacterium of genus Pseudomonas on sowing characteristics of tomato seeds