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Документ Biodestruction of phenol by non-pathogenic bacteria of the genus Pseudomonas(Odessa I. I. Mechnikova National University, 2017) Horshkova, Olena H.; Voliuvach, Olha V.; Bebyk, V. V.; Kolomiets, O. G.; Lagovskaya, L. S.; Khadzhy, V. D.; Волювач, Ольга Вячеславовна; Волювач, Ольга В'ячеславівна; Горшкова, Олена Георгіївна; Горшкова, Елена ГеоргиевнаIt has been experimentally established that two strains of bacteria genus Pseudomonas, isolated from the marine environment and identified by the fatty acid composition of their cellular lipids as P. fluorescens ONU328, P. maltophilia ONU329, are not pathogenic and have a high phenol-oxidizing ability. Bacteria are cultured for two days at a temperature of 30 °C on MPA, further bacterial cells are suspended in the mineral medium M-9, and in the optimum amount is introduced into the contaminated water containing up to 300 mg/l phenol. It was established that the strain of P. maltophilia possessed a greater phenol-oxidizing activity than the strain P. fluorescens. When water was treated with strain P. maltophilia with a culture concentration of 3.5×104 CFU/ml, deep purification from phenol was observed on 34 day, and with an increase in the culture concentration to 7.5×105 CFU /ml on 18 day, t=30 °C.Документ Biomarker values of nonpathenous microorganisms with polyfunctional biotechnological properties(Odessa I. I. Mechnikova National University, 2017) Horshkova, Olena H.; Voliuvach, Olha V.; Kolomiets, O. G.; Bebyk, V. V.; Khadzhy, V. D.; Lagovskaya, L. S.; Волювач, Ольга В'ячеславівна; Волювач, Ольга Вячеславовна; Горшкова, Елена Георгиевна; Горшкова, Олена ГеоргіївнаIsolated from the oil-contaminated soil of Zmeiniy іsland and the marine environment are four non-pathogenic biochemically active strains of microorganisms identified by the fatty acid composition of their cell lipids as Microbacterium barkeri OZ-3, Bacillus megaterium OZ-5 (isolated from soil), Pseudomonas fluorescens ONU-328, Pseudomonas maltophilia ONU-329 (separated from the marine environment). The strains possess polyfunctional biotechnological properties: sorptionaccumulating ability relative to heavy metal ions, oxidizing activity with respect to petroleum products, anionic surfactants and phenolic compounds. Biomarker values for distinguishing the strains of microorganisms studied (Microbacterium barkeri OZ-3, Bacillus megaterium OZ-5, Pseudomonas fluorescens ONU-328, Pseudomonas maltophilia ONU-329) are proposed at the species level.Документ Cellular fatty acid composition of Aeromonas genus – destructor of aromatic xenobiotics(2019) Gudzenko, Tetiana V.; Horshkova, Olena H.; Korotaieva, Nadiia V.; Voliuvach, Olha V.; Ostapchuk, Andriy M.; Ivanytsia, Volodymyr O.; Гудзенко, Тетяна Василівна; Горшкова, Олена Георгіївна; Коротаєва, Надія Володимирівна; Волювач, Ольга В'ячеславівна; Іваниця, Володимир ОлексійовичThe aim of this study was a determination of the fatty acid composition of cellular lipids and identification of the strains, isolated from the wastewater of pharmaceutical production, – the destructor of aromatic xenobiotics. The phenotypic characteristics and cellular fatty acid (FA) composition confirmed the strain belonging to the Aeromonas ichthiosmia with the similarity index of library data MIDI Sherlock - 0.564. Analysis of the cellular FA composition of the strain Aeromonas ichthiosmia ONU 552 was carried out using the MIDI Sherlock microorganism identification system based on the gas chromatograph Agilent 7890. Chromatographic analysis showed that the fatty acid profile of the strain Aeromonas ichthiosmia ONU 552 contains 26 fatty acids with the total number of carbon atoms from 10 to 18. 85.27% of saturated and unsaturated fatty acids had unbranched structure. The total content of unsaturated fatty acids – 16:1 w7c/16:1 w6c, 18:1 w7c, 16:1 w7c alcohol, 17:1 w8c, 17:1 w6c, 16:1 w5c, was 50% of the total fatty acid pool. Less than 1.5% branched fatty acids were predominantly in the iso form: 13:0 iso (0.20%); 15:0 iso (0.97%); 17:1 iso w9c (1.35%), 17:0 iso (1.49%); in the anteiso form, only one acid 17:0 (0.27%) was identified. It was shown that the characteristic of the fatty acid composition of the strain Aeromonas ichthiosmia ONU 552 – the destructor of aromatic xenobiotics, was the presence of hydroxyacids 12:0 3OH, 15:0 3OH, 15:0 iso 3OH and dominance of hexadecanoic (16:0) and hexadecenoic (16:1 w7c/16:1 w6c) of fatty acids.Документ Detection of markers in the fat -acid profile of bacillus subtilis ONU 551 - destructor of disinfectants(2018) Horshkova, Olena H.; Voliuvach, Olha V.; Gudzenko, Tetiana V.; Chernyshova, Myroslava O.; Nester, Oleksandr O.; Волювач, Ольга Вячеславовна; Волювач, Ольга В'ячеславівна; Горшкова, Елена Георгиевна; Горшкова, Олена ГеоргіївнаTo increase the efficiency of the work of medical institutions and pharmaceutical production facilities, where a large number of phenolic and other hardly oxidizable compounds are found in the sewage waters, we recommend using strain Bacillus subtilis ONU551 (F13) to purify this kind of effluent. The strain was isolated in 2017 from wastewater produced by Ukrainian pharmaceutical products, and does not lose destructive properties with respect to aromatic xenobiotics in the presence of pathogenic microbiota - E. coli, K. pneumoniae, S. moscow, P. aeruginosa.Документ Detection of markers in the fat –acid profile of Bacillus subtilis ONU 551 - destructor of disinfectants(2018) Horshkova, Olena H.; Voliuvach, Olha V.; Gudzenko, Tetiana V.; Chernyshova, Myroslava O.; Nester, A. A.; Волювач, Ольга В'ячеславівна; Волювач, Ольга Вячеславовна; Горшкова, Елена Георгиевна; Горшкова, Олена Георгіївна; Гудзенко, Тетяна Василівна; Гудзенко, Татьяна ВасильевнаTo increase the efficiency of the work of medical institutions and pharmaceutical production facilities, where a large number of phenolic and other hardly oxidizable compounds are found in the sewage waters, we recommend using strain Bacillus subtilis ONU551 (F13) to purify this kind of effluentДокумент Efficiency of purification of water from cyclic aromatic xenobiotics with strains P. fluorescens ONU328 and P. maltophilia ONU329(Одеський національний університет імені І. І. Мечникова, 2018) Horshkova, Olena H.; Voliuvach, Olha V.; Tkachenko, A.; Pihteeva, A.; Smazchuk, O.; Haidarzhi, A.; Волювач, Ольга Вячеславовна; Волювач, Ольга В'ячеславівна; Горшкова, Олена Георгіївна; Горшкова, Елена ГеоргиевнаAbstract. It has been experimentally established that non-pathogenic strains of bacteria of the genus Pseudomonas, identified by the fatty acid composition of their cellular lipids as P. fluorescens ONU328, P. maltophilia ONU329 (isolated from the marine environment), have oxidizing ability for cyclic aromatic xenobiotics (phenol, N-cetylpyridinium bromide). The high phenol-oxidizing ability of the investigated strains of microorganisms is proved. When water of bacterial cells in the amount of 7.5 x 105 CFU/ ml is introduced into phenol contaminated (at a concentration of 300 mg / l), the degree of water purification from phenol is 100% on day 18 when using as a biodestructor strain P. maltophilia ONU329 and 22 days - when using strain P. fluorescens ONU328. It has been experimentally confirmed that when the free cells of bacteria of P. fluorescens ONU328 or P. maltophilia ONU329 strains in the amount of 5.5x104 CFU/ ml are introduced once into the contaminated water, the purification rate of water from N-cetylpyridinium bromide at a concentration of 20 mg / l reaches 50.7% for the 4th day and 55.7% for the 6th day, respectively. With the repeated introduction of fresh portions of microorganism-destructors or use of higher starting doses (5.5x10 CFU/ ml), the water purification rate from N-CPB is 98.0-98.5%.Документ Fatty acids composition of Bacillus subtilis ONU551 lipids(2019) Gudzenko, Tetiana V.; Voliuvach, Olha V.; Horshkova, Olena H.; Ostapchuk, Andriy M.; Ivanytsia, Volodymyr O.; Гудзенко, Тетяна Василівна; Волювач, Ольга В'ячеславівна; Горшкова, Олена Георгіївна; Іваниця, Володимир ОлексійовичThe aim of the study was to determine the cellular lipids fatty acid composition for identification of the Bacillus subtilis ONU 551 strain bacteria, which is a phenol destructor. Fatty acids analysis of B. subtilis ONU 551 strain was performed using an automatic system for microorganisms’ identification MIDI Sherlock (MIDI , USA ) based on gas chromatograph Agilent 7890. Chromatograms analysis showed that the fatty acid spectrum of the strain B. subtilis ONU 551 consisted predominately of branched structural isomers of saturated acids: 13-methyltetradecanoic (15:0 iso; 34.72%) and 12-methyltetradecanoic (15:0 anteiso; 33.72%) acids. The total content of the branched saturated fatty acids was 88.16% – 14:0 iso (0.52%), 15:0 iso (34.72%), 15:0 anteiso (33.72%), 16:0 iso (1.85%), 17:0 iso (7.11%), 17:0 anteiso (10.24%). The saturated fatty acids of the normal structure were also detected – 12:0 (0.36%), 14:0 (0.28%), 16:0 (1.30%). No 2- and 3-hydroxy acids and no cyclic fatty acids were detected in the fatty acid profile of B. subtilis ONU 551 strain. Unsaturated fatty acid isomers – 15:1 w5c (1.85%), 16:1 w11c (1.21%), 16:1 w7c alcohol (1.08%), 17:1 iso w10c (3.18%), Σ17:1 iso I/anteiso B (2.57%) were shown to be the distinctive biomarkers of the B. subtilis ONU 551 strain. According to the fatty acid profile analysis with MIDI Sherlock system, the studied strain was identified as Bacillus subtilis with high level of similarity index (0.563).Документ Fatty acids composition of microbacterium genus bacteria-destructors of oil hydrocarbons(2016) Horshkova, Olena H.; Korotaieva, Nadiia V.; Ostapchuk, A. M.; Voliuvach, Olha V.; Gudzenko, Tetiana V.; Горшкова, Олена Георгіївна; Коротаєва, Надія Володимирівна; Волювач, Ольга В'ячеславівна; Гудзенко, Тетяна ВасилівнаTo conduct an identification of microorganisms – destructors of oil hydrocarbons – based on their fatty acid composition. This is one of the chemotaxonomic characteristics that correlate with bacterial molecular genetic features. Fatty acids analysis of cells of bacteria was carried out using automatic system of microorganisms’ identification MIDI Sherlock (MIDI, USA) based on gas chromatograph Agilent 7890. For fatty acids profiles test culture were characterized by С15:0 anteiso – 44.26–46.36 %, С17:0 anteiso – 27.04– 28.03 %, С16:0 iso – 14.49–16.9 %, С15:0 iso – 5.51– 8.58 %, С17:0 iso – 1.67–2.4 %. Isomers С13:0 anteiso, С14:0 iso, С18:0 iso, С19:0 anteiso identified smaller amounts and ranged from 0.05 to 0.72 % of the total peaks square. It demonstrates the presence of saturated isomers С14:0, С16:0, С17:0 and С18:0, which composition ranged from 0.05 to 1.02 %. By phenotypic characteristics and the composition of fatty acids investigated culture were assigned to type Microbacterium barkeri. Similarity index of fatty acid profiles of the library is 0.685 for strain Microbacterium barkeri ОZ-2 and 0.942, for strain Microbacterium barkeri ОZ-3.Документ Features of fatty strength profile of strain Brevibacillus centrosporus F14 – destructor of phenolic compounds(НАН України. Ін-т біохімії ім. О.В. Палладіна, 2018) Horshkova, Olena H.; Shtenikov, Mykola D.; Korotaieva, Nadiia V.; Voliuvach, Olha V.; Горшкова, Олена Георгіївна; Штеніков, Микола Дмитрович; Коротаєва, Надія Володимирівна; Волювач, Ольга В'ячеславівнаClose-bodied microorganisms are clearly delineated by the presence and percentage content of their cellular lipids of fatty acids. Particular attention is paid to the search for new and identification of non-pathogenic microorganisms intended for the purification of effluents from the production of pharmaceuticals, medical institutions with the predominant content of highly toxic phenolic compounds in them. The aim of the work is to establish the characteristics of the fatty acid profile of the nonpathogenic strain Brevibacillus centrosporus F14, a destructor of phenolic compounds isolated from sewage from Ukrainian pharmaceutical products.Документ Features of the fatty acid profile of non -pathogenic strains aeromonas ichthiosmia ONU 552, bacillus subtilis ONU 551-destructors of phenolic compounds(2019) Radysh, N. B.; Lebedeva, E. V.; Voliuvach, Olha V.; Horshkova, Olena H.; Gudzenko, Tetiana V.; Волювач, Ольга В'ячеславівна; Горшкова, Олена Георгіївна; Гудзенко, Тетяна Василівна; Волювач, Ольга Вячеславовна; Горшкова, Елена Георгиевна; Гудзенко, Татьяна ВасильевнаThe search for new environmentally safe biological products designed to clean the environment from toxic organic pollutants remains a pressing problem of our time.Документ Formation of the multispecies biofilm of phenol bacteria-destructors on natural and synthetic carriers in a biofilter(2019) Chaban, M. M.; Horshkova, Olena H.; Voliuvach, Olha V.; Gudzenko, Tetiana V.; Konup, Ihor P.; Beliaeva, Tamara O.; Galkin, Mykola B.; Горшкова, Олена Георгіївна; Волювач, Ольга В'ячеславівна; Гудзенко, Тетяна Василівна; Конуп, Ігор Петрович; Галкін, Микола БорисовичFluorescent microscopy using acridine orange dye confirmed that bacteria phenol- destructors used for water purification formed biofilm on the biofilter media of different nature - zeolite, flaps mussels, synthetic carrier type VII, charcoal, peat, ceramic tubes, sand.Документ Method of eliminating oil film pollution on the water area(2015) Horshkova, Olena H.; Voliuvach, Olha V.; Piddubnaya, S.; Pototskaya, A.; Kovtalo, M. S.; Tkachenko, A.; Волювач, Ольга В'ячеславівна; Волювач, Ольга Вячеславовна; Горшкова, Елена Георгиевна; Горшкова, Олена ГеоргіївнаThe method of using the biopreparation – a sorbent and destructor of oil hydrocarbons which block oil pollution of the aquatic environment in the shortest possible time prevents spreading and eliminates it with minimum ecological loss.Документ Microbiological method of purification of water from phenol association of non-pathogenic bacteria straps of the genus Pseudomonas(Одеський національний університет імені І. І. Мечникова, 2018) Horshkova, Olena H.; Voliuvach, Olha V.; Samofatov, Mykhailo I.; Tkachenko, A.; Волювач, Ольга Вячеславовна; Волювач, Ольга В'ячеславівна; Горшкова, Елена Георгиевна; Горшкова, Олена ГеоргіївнаA microbiological method for purifying water from phenol has been developed, which consists in using phenol-bacterial association of strains of non-pathogenic bacteria of the genus Pseudomonas: P. cepacia ONU-327 and P. fluorescens ONU- 328, taken in a volume ratio of 1: 1. When using a biopreparation on the basis of the association of the bacterial strains under study, the oxidation of phenol is accelerated 2.2 times as compared to the treatment of phenol-containing water with monocultures. Deep purification of water from phenol with a concentration of 300 mg/l occurs within 10 days with a single injection of bacterial cells in an amount of 7.5x105 CFU/ml, pH 7, a temperature of 28-30 °C. It has been established that the bacterial association of strains of P. cepacia ONU-327 and P. fluorescens ONU-328 is capable not only of effective destruction of phenol, but also possesses a high sorption-accumulating potential for ions of heavy metals [Pb (II), Zn (II) , Cu (II), Cr (V)], which reveals the prospects of its use in the biopreparation for the purification of multicomponent pharmacies, with the predominant content of phenolic contaminants.Документ Peculiarities of chemical composition of phenol -oxidizing stra in cells aeromonas ichthiosmia ONU 552(2018) Horshkova, Olena H.; Voliuvach, Olha V.; Gudzenko, Tetiana V.; Chernyshova, M. O.; Nester, A. A.; Горшкова, Олена Георгіївна; Волювач, Ольга В'ячеславівна; Гудзенко, Тетяна Василівна; Горшкова, Елена Георгиевна; Гудзенко, Татьяна Васильевна; Волювач, Ольга ВячеславовнаThe chemical composition of cells of strains of microorganisms is necessary for their chemodifferentiation. This information can be used to determine the generic (species) belonging to a strain in ecological, biotechnological and infectious communities of microorganisms for medical diagnosis or for further use in the biotechnological direction. Of particular interest is the introduction of advances in biotechnology techniques based on the use of non-pathogenic strains of microorganisms immobilized on synthetic carriers, in the practice of treating effluent from medical institutions with a high content of phenolic contaminants.Документ Peculiarities of chemical composition of phenol -oxidizing strain cells aeromonas ichthiosmia ONU 552(2018) Horshkova, Olena H.; Voliuvach, Olha V.; Gudzenko, Tetiana V.; Chernyshova, Myroslava O.; Nester, Oleksandr O.; Волювач, Ольга В'ячеславівна; Волювач, Ольга Вячеславовна; Горшкова, Олена Георгіївна; Горшкова, Елена ГеоргиевнаThe chemical composition of cells of strains of microorganisms is necessary for their chemodifferentiation. This information can be used to determine the generic (species) belonging to a strain in ecological, biotechnological and infectious communities of microorganisms for medical diagnosis or for further use in the biotechnological direction. Of particular interest is the introduction of advances in biotechnology techniques based on the use of non-pathogenic strains of microorganisms immobilized on synthetic carriers, in the practice of treating effluent from medical institutions with a high content of phenolic contaminants.Документ Peculiarities of chemical composition of phenol -oxidizing straincells Aeromonas ichthiosmia ONU 552(2018) Horshkova, Olena H.; Voliuvach, Olha V.; Gudzenko, Tetiana V.; Chernyshova, Myroslava O.; Nester, A. A.; Волювач, Ольга Вячеславовна; Волювач, Ольга В'ячеславівна; Горшкова, Елена Георгиевна; Горшкова, Олена ГеоргіївнаThe chemical composition of cells of strains of microorganisms is necessary for their chemodifferentiation.Документ Phenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strain(2020) Gudzenko, Tetiana V.; Voliuvach, Olha V.; Horshkova, Olena H.; Ostapchuk, Andriy M.; Ivanytsia, Volodymyr O.; Гудзенко, Тетяна Василівна; Волювач, Ольга В'ячеславівна; Горшкова, Олена Георгіївна; Іваниця, Володимир ОлексійовичBiotechnological treatment of enterprises wastewater, which contains toxic phenolic compounds, is the most acceptable method today. Therefore, the search for new non-pathogenic biochemically active microorganisms - destructors of phenol and their identification is relevant. The aim of this study was to determine phenol-oxidizing activity and the fatty acid profile of Brevibacillus centrosporus F14 – destructor of phenol, isolated from pharmaceutical plant wastewater. Fatty acids analysis of the microorganism culture was carried out using automatic system of microorganisms identification MIDI Sherlock based on gas chromatograph Agilent 7890. The phenol-oxidizing activity of bacteria was determined by photometric method according to the degree of phenol extraction from the water. It was experimentally confirmed that Brevibacillus sp. F14 strain had a phenol-destructive activity. When phenol-containing water was treated with Brevibacillus sp. F14 cells, phenol concentration in the water decreased from 200.0 ± 12.0 mg/l to 6.8 ± 0.8 mg/l during 15 days of exposure. Chromatographic analysis showed that saturated fatty acids i-14:0 (14.9%), i-15:0 (14.8%), a-15:0 (34.9%), i-16:0 (11.10%) dominated in the strain’s fatty acids profile. The total content of the branched saturated fatty acids i-14:0, i-15:0, a-15: 0, i-16: 0, i-17: 0, a-17:0, Σ(a-17:1/i-17:1) was 85.0% and that of saturated and unsaturated fatty acids with the normal structure – 7% of the total fatty acids pool. The distinction of Brevibacillus centrosporus F14 strain’s fatty acid profile from other bacteria of Brevibacillus genus was the presence of more i-14:0 (14.85%), i-16:0 (11.10%) fatty acids and 16: 1 w7c alcohol (7.71%), as well as the minor content of fatty acids markers 16:0 N alcohol (0.60%), 18:3 w6c (0.4%) and Σ(a-17:1/i-17:1) (1.94%). According to the phenotypic characteristics and fatty acid composition, the tested phenol-oxidizing strain belongs to Brevibacillus centrosporus species. The index of Brevibacillus centrosporus F14 strain’s fatty acid profile similarity with the library was 0.645.Документ Screening of marine microorganisms resistant toaction of deca (ethylene glycol ) monooctylphenol ether(2017) Horshkova, Olena H.; Gudzenko, Tetiana V.; Smazchuk, O.; Pihteeva, A.; Haidarzhi, A.; Voliuvach, Olha V.; Волювач, Ольга В'ячеславівна; Волювач, Ольга Вячеславовна; Горшкова, Елена Георгиевна; Горшкова, Олена Георгіївна; Гудзенко, Татьяна Васильевна; Гудзенко, Тетяна ВасилівнаSynthetic surfactants are a part of all domestic and most industrial wastewater. 95-98% of the total amount of detergents used in Ukraine are synthetic detergents based on surfactant mixtures, which, as a rule, are characterized by low biological decomposition and because of their chemical nature have a negative impact on water bodiesДокумент Strains Pseudomonas fluorescens ONU 541, Bacillus megaterium ONU 542 are promising producers of surface-active met abolites and destructors of petroleum products(2017) Horshkova, Olena H.; Voliuvach, Olha V.; Shtenikov, Mykola D.; Gudzenko, Tetiana V.; Волювач, Ольга Вячеславовна; Волювач, Ольга В'ячеславівна; Горшкова, Елена Георгиевна; Горшкова, Олена ГеоргіївнаMethods for eliminating oil pollution, based on their decomposition by non-pathogenic microorganisms, are recognized as effective and ecologically safeДокумент Strains pseudomonas fluorescens ONU 541, bacillus megaterium ONU 542 are promising producers of surface-active metabolites and destructors of petroleum products(2017) Horshkova, Olena H.; Voliuvach, Olha V.; Shtenikov, M.; Gudzenko, Tetiana V.; Горшкова, Олена Георгіївна; Волювач, Ольга В'ячеславівна; Гудзенко, Тетяна Василівна; Горшкова, Елена Георгиевна; Волювач, Ольга Вячеславовна; Гудзенко, Татьяна ВасильевнаMethods for eliminating oil pollution, based on their decomposition by non-pathogenic microorganisms, are recognized as effective and ecologically safe. The degree of purification is increased in the case of the production of biosurfactants by microorganisms. The urgent task of ecobiotechnology remains the search for new oil-oxidizing microorganisms capable of producing biosurfactants on a cheap nutrient medium. The purpose of the work is to screen microorganisms isolated from the oil-contaminated soil of Zmiiny Island, promising in the biotechnological plan for the production of surface-active metabolites and the destruction of petroleum hydrocarbons.