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Документ Ability of Lactobacillus plantarum ONU 12 and Bacillus megaterium ONU 484 to stimulate growth of wheat seedlings and to form biofilms(Одеський національний університет імені І. І. Мечникова, 2018) Tverdokhlib, Veronika S.; Limanska, Nataliia V.; Krylova, Kateryna D.; Ivanytsia, Volodymyr O.; Твердохліб, Вероніка Сергіївна; Ліманська, Наталія Вікторівна; Крилова, Катерина Дмитрівна; Іваниця, Володимир Олексійович; Твердохлеб, Вероника Сергеевна; Лиманская, Наталия Викторовна; Крылова, Екатерина Дмитриевна; Иваница, Владимир АлексеевичDevelopment of biological preparations for organic agriculture should include the study of interactions of microorganisms – the components of biopreparations, with representatives of natural microbiota of agrocoenoses. As a microorganism – typical representative of epiphytic and soil microbiota, species Bacillus megaterium has been selected. Aim. The study of effect of Lactobacillus plantarum ONU 12 and Bacillus megaterium ОNU 484 on germination and growth of wheat seedlings. Materials and Methods. Seeds of wheat Triticum aestivum L. were inoculated with suspensions of bacteria B. megaterium ОНУ 484, L. plantarum ОНУ 12 and their mixture was germinated in hydroponics and soil under green house conditions. Germination of seeds, mean length of roots and height of seedlings were compared. Capability of these microorganisms to form biofilms was studied: inoculated seedlings were dyed with 0.1% acridin orange and observed with a magnification х600. Results. Simultaneous inoculation with bacteria L. plantarum ONU 12 and with the representatives of soil microbiota B. megaterium ОNU 484 didn’t decrease the stimulation effect of lactobacilli. Opposite, the highest stimulation effect on plants both in hydroponics and in soil caused the treatments with the mixture L. plantarum ONU 12 + B. megaterium ONU 484 and with the strain B. megaterium ONU 484 alone. In hydroponics mean length of roots increased in 8.0 – 16.9%, mean height of seedlings – in 8.8 – 24.3%. In soil germination of seeds increased in 7.0%, mean height of seedlings – in 7.6%, mean root length – in 13.1%. Lactobacilli and bacilli in the mixture were able to form biofilms with a developed matrix. Conclusions. In presence of representative of soil microbiota B. megaterium ONU 484 phytostimulation the activity of lactobacilli increased. Strain B. megaterium ONU484 also showed stimulation activity when applied separately.Документ Alcaligenes faecalis onu 452 as an agent of bacterial biocontrol against Rhizobium radiobacter c58(2015) Kruchanova, A.; Korotaieva, Nadiia V.; Limanska, Nataliia V.; Ліманська, Наталія Вікторівна; Коротаєва, Надія ВолодимирівнаCrown gall of dicotyledonous plants causes uncontrolled tumor formation, and is among the most damaging diseases to the rural economy. The main pathogens of the crown gall are bacteria Rhizobium vitis and R. Radiobacter. (Szegedi et al. 2005, Kawaguchi and Inoue 2009).Документ AMOUNT OF ENDOPHYTIC MICROBIOTA IN GRAPEVINE SHOOTS(Одеський національний університет, 2016) Marynova, І. І.; Ivanitsa, V. Yu.; Limanska, Nataliia V.; Маринова, І. І.; Іваніца, В. Ю.; Ліманська, Наталія Вікторівна; Маринова, И. И.; Иваница, В. Ю.; Лиманская, Наталия ВикторовнаThe aim of the investigation was to evaluate the amount of grapevine endophytic microbiota using different nutritional media. Materials and methods. Endophytic microbiota from wooden shoots of grapevine Vitis vinifera L. cv Arkadia was isolated on the eight nutritional media with different compositions. Results. The highest amounts of bacteria were isolated on YEM, YMA and TY nutritional media. The distinctive features of YMA and YEM media was the presence of mannitol, and of TY – the increased concentration of yeast extract. Conclusion. Amount of microbiota representatives from grapevine shoots reached from (6.4±0.3) х 104 to (2.0±0.4) х 107 CFU/сm3 depending on the nutritional medium and decreased from October to December at least in one range.Документ Antagonistic activity of Lactobacillus plantarum against crown gall agent(Одеський національний університет імені І. І. Мечникова, 2017) Nevynna, Tetiana V.; Limanska, Nataliia V.; Невинна, Тетяна Віталіївна; Ліманська, Наталія ВікторівнаLactobacilli are known for a wide range of antagonistic properties. Therefore application of these bacteria for protecting plants against pathogens is a perspective trend. The aim of this work was to study the effect of L. plantarum cultures against agent of crown gall R. radiobacter. The strains of lactobacilli isolated from grape must were initially investigated for their morphological and growth characteristics. Morphology of bacteria and colonies, lactic fermentation indicated that the studied microorganisms belonged to lactic acid bacteria. To confirm this, the polymerase chain reaction with primers for speciesspecific gene site plnN of L. plantarum was performed. It was found that all investigated strains possessed gene plnN. Antagonistic properties of 15 strains of L. plantarum first were studied in experiments in vitro by well-diffusion method. All strains inhibited the growth of pathogens.Документ Antagonistic activity of Lactobacillus plantarum against crown gall agent(2013) Limanska, Nataliia V.; Korotaieva, Nadiia V.; Biscola, Vanessa; Ivanytsia, Tetіana V.; Basiul, Olena V.; Choiset, Yvan; Merlich, Andrii H.; Kondratiuk, Tetiana V.; Yamborko, Hanna V.; Nazarenko, Hanna M.; Chobert, Jean-Marc; Ivanytsia, Volodymyr O.; Haertle, Thomas; Ямборко, Ганна Валентинівна; Ліманська, Наталія Вікторівна; Коротаєва, Надія Володимирівна; Біскола, Ванесса; Іваниця, Тетяна Володимирівна; Басюл, Олена Владленівна; Мерліч, Андрій Геннадійович; Кондратюк, Тетяна В.; Назаренко, Ганна Миколаївна; Іваниця, Володимир ОлексійовичThe aim of this work was to study the antagonistic effect of Lactobacillus planlarum on phytopathogenic agrobacteria. Lactobacilli isolated from various plant sources have been identified by microbiological and molecular biological methods and a LAB collection has been formed. Physiological, biochemical, molecular biological and technological properties of the isolated bacteria were characterized.Документ Application of lactic acid bacteria in agriculture: perspectives and challenges(Odesa I. I. Mechnikov National University, 2016) Limanska, Nataliia V.; Basiul, Olena V.; Kutiian, D.; Saparova, O.; Kononiuk, Yu. V.; Nevynna, T. V.; Arzamasova, A.; Reva, K.; Ivanytsia, Volodymyr O.; Sokol, D.; Ліманська, Наталія Вікторівна; Іваниця, Володимир ОлексійовичApplication of lactic acid bacteria for plant protection and stimulation of plant growth started in 1980s with works of Visser et al. (1986) and Higa et al. (1989). Nevertheless, till now there is no clear scientific explanation of the mechanism of growth stimulation. We found out that lactobacilli can increase growth of stems, roots and stimulate the germination of tomatoes, garden cress and wheat (Limanska, 2013; unpublished data). But it also was shown that in consortia the positive capabilities of Lactobacillus plantarum increased. What is the mechanism of such beneficial co-existence? It is still unknown. Only one work of Goffin et al. (2010) described the possible synthesis of plant hormones or hormone-like compounds of L. plantarum. The mechanism of such mutual effects could be based on cell-cell interactions. Induction of bacterial activity of lactobacilli as the result of interaction with microorganisms of other species is known for the synthesis of bacteriocins (Rojo-Bezares, 2007). It is possible that the similar mechanism exists for the synthesis of stimulating compounds.Документ Biofilm formation and genetic diversity of Lactobacillus plantarum strains originated from France and Ukraine(2019) Limanska, Nataliia V.; Merlich, Andrii H.; Galkin, Mykola B.; Vasylieva, Nataliia Yu.; Choiset, Yvan; Ivanytsia, Tetіana V.; Zlatohurska, Maryna; Ivanytsia, Volodymyr O.; Chobert, Jean-Marc; Ліманська, Наталія Вікторівна; Мерліч, Андрій Геннадійович; Галкін, Микола Борисович; Васильєва, Наталія Юріївна; Іваниця, Тетяна Володимирівна; Іваниця, Володимир ОлексійовичEvaluation of Lactobacillus plantarum strains perspective for plant protection or fermentation of food of plant origin should include tests on abilities to attach to plant surfaces and to form biofilms. Comparing RAPD-profiles with these properties and also with geographical origin of the strains is necessary to find out the possibility of rapid selection of the strains and tracking their dissemination. Biofilm formation and RAPD-PCR profiles of 30 strains of L. plantarum isolated from grape must and pickles originated from France and Ukraine were compared. All isolates were able to attach to Lepidium sativum seedlings and form biofilms of different types. Amount of attached cells varied from (7.8 ± 0.2) x 104 to (2.1 ± 0.3) x 103 CFU/cm2. The most developed biofilms were formed on shoots, leaves, seed coat shells. On roots this ability was strain dependent: the majority of French isolates (77.0%) formed separated microcolonies without developed matrix layer. Among Ukrainian isolates, 47.1% of the strains showed the same tendency, and in 41.2% of the strains individual attached cells without formation of microcolonies were observed. The level of initial attachment of bacterial cells to roots did not coincide with the level of subsequent formation of biofilms. Gene plnA was detected in 28.1% of isolates. All plnA possessing strains except the one formed microcolonies or biofilms with developed matrix. Lactobacilli could eliminate the biofilms of Agrobacterium tumefaciens pJZ from tomato roots in 75.0 – 100% of tested samples depending on a strain and method of inoculation. No clear association between the geographical origin of the L. plantarum strains and clustering by RAPD-PCR analysis was found. Two strains with high level of biofilm formation were grouped in one subcluster.Документ Biofilm formation of Lactobacillus plantarum on plant surfaces(Odesa I. I. Mechnikov National University, 2016) Sokol, D.; Merlich, Andrii H.; Marynova, I. V.; Galkin, Mykola B.; Limanska, Nataliia V.; Мерліч, Андрій Геннадійович; Галкін, Микола Борисович; Ліманська, Наталія ВікторівнаAttachment of Lactobacillus plantarum to plant surfaces and biofilm formation are poorly understood. To create a biotechnology for the plant protection from pathogens the study of mechanisms of adhesion of lactobacilli and selection of the strains with the high ability to biofilm formation is needed.Документ Biological properties of nitrogen-fixing bacteria isolated from rhizosphere(Одеський національний університет імені І. І. Мечникова, 2018) Rudenko, A.; Korotaieva, Nadiia V.; Limanska, Nataliia V.; Коротаєва, Надія Володимирівна; Ліманська, Наталія ВікторівнаNirogen-fixing bacteria were isolated from rhizosphere of grape and characterized by the main biological properties: morphology, ability to grow on nutrient media, fatty-acid analysis. Strain was identified as a candidate to Azotobacter sp.Документ CLOSTRIDIUM PERFRINGENS PHAGE INFLUENCE ON ESTABLISHED HUMAN CELLS CULTURE HEP-2 IN A LOGARITHMIC PHASE OF GROWTH(Печатный дом, 2007) Horshkova, Olena H.; Ivanytsia, Tetіana V.; Galkin, Mykola B.; Zaharkina, T.; Narceva, O.; Unguryan, A.; Chaban, I.; Gudzenko, Tetiana V.; Filipova, Tetiana O.; Chanishvili, N.; Burbutashvili, T.; Limanska, Nataliia V.; Горшкова, Елена Георгиевна; Иваница, Татьяна Владимировна; Галкин, Николай Борисович; Захаркіна, Т.; Нарцева, О.; Унгурян, А.; Чабан, И.; Гудзенко, Татьяна Васильевна; Філіпова, Тетяна Олегівна; Чанишвили, Н.; Бурбуташвили, Т.; Лиманская, Наталия Викторовна; Горшкова, Олена Георгіївна; Гудзенко, Тетяна Василівна; Филиппова, Татьяна Олеговна; Іваниця, Тетяна Володимирівна; Галкін, Микола Борисович; Ліманська, Наталія ВікторівнаDuring the history of bacteriophages there were both great interest to them in a period of their first description and almost complete oblivion in 60-80 years. In 30-40 years of XX century when antibiotics had not been discovered the cheap use of bacteriophages was successfully applied in curing and prevention of many infections.Документ Complex investigations of plant microbiota representatives(Одеський національний університет імені І. І. Мечникова, 2018) Kononiuk, Yu. V.; Marynova, I. V.; Adarma, N. Yu.; Stepaniuk, G.; Bratkevich, V.; Tverdokhlib, V. S.; Basiul, Olena V.; Limanska, Nataliia V.; Ліманська, Наталія ВікторівнаPhytopathogenic bacteria - crown gall agents - were isolated from grape plants. Antagonistic bacteria - also plant dwellers - were used to struggle these pathogens. Lactobacillus plantarum effectively inhibited crown gall agents and survived in soil at least for 45 days. Lactobacilli could interfere pathogenesis of the disease on a step of pathogen attachment. Together with Bacillus megaterium, lactobacilli showed stimulation effect on plant growth.Документ Detection of crown gall agents on Ukrainian vineyards by PCR(Odesa I.I. Mechnykov university, 2009) Limanska, Nataliia V.; Ivanytsia, Volodymyr O.; Milkus, Boris; Zhunko, Inna D.; Konup, Lyudmila; Gavrik, Alla; Ліманська, Наталія Вікторівна; Іваниця, Володимир Олексійович; Жунько, Інна ДавидівнаCrown gall of grape is one of the most serious diseases in commercial nurseries of many grape-growing countries. On young vineyards of susceptible cultivars up to 75% of plants may die from galls surrounding trunks and interfering the normal water and nutrients supply (Negrul, 1951, Burr, 1999). Crown gall agents (Rhizobium vitis and R. radiobacter (Agrobacterium vitis, A. tumefaciens)) survive in grapevine xylem and are transmitted by vegetative propagation (Lehoczky, 1968). In many cases infected plants remain symptomless for a long time, allowing to select planting material from infected vineyards (Burr, 1984). Use of such planting material results in further spread of pathogens and consequent losses in viticulture.Документ DETECTION OF PLANTARICIN GENES IN STRAINS OF LACTOBACILLUS PLANTARUM – ANTAGONISTS OF PHYTOPATHOGENIC BACTERIA(Одеський національний університет імені І. І. Мечникова, 2015) Limanska, Nataliia V.; Babenko, Dmytro O.; Yamborko, Hanna V.; Ivanytsia, Volodymyr O.; Ліманська, Наталія Вікторівна; Бабенко, Дмитро Олександрович; Ямборко, Ганна Валентинівна; Іваниця, Володимир Олексійович; Лиманская, Наталия Викторовна; Бабенко, Дмитрий Александрович; Ямборко, Анна Валентиновна; Иваница, Владимир АлексеевичThe aim of investigation was to detect the presence of genes responsible for bacteriocin synthesis in strains of Lactobacillus plantarum with the clear antagonistic effect against the gram-negative phytopathogens. Methods. To reveal eleven genes involved in plantaricin synthesis the polymerase chain reaction was used. To test the ability to synthesize bacteriocins as antagonistic compounds the experiments with the lawns of test-strains Listeria ivanovii INRA, Rhizobium radiobacter C58, Ralstonia solanacearum B-1109-UCM, Erwinia carotovora ZM1, Rhizobium vitis OНУ 389, R. vitis OНУ 388, R. vitis 379 and R. rhizogenes 15834 were used. Results. In genomes of the tested L. plantarum strains the genes plnD, plnEF, plnG, plnI, plnN were present, but the genes plnA, plnB, plnC, plnW were not revealed. Applying the cultural liquids of lactobacilli on the lawns of the test-strains has shown that the cell-free cultural liquid with the initially low pH (4.1–4.3) caused the zones of growth inhibition on the lawns of all test-strains. The neutralized cell-free cultural liquid did not affect the growth of the test-strains. Conclusion. Although the tested strains L. plantarum ОNU 87, L. plantarum ОNU 206 and L. plantarum ОNU 991 possessed some genes of plantaricin regulon, in the investigations in vitro they caused the inhibition of the phytopathogens and listerias due to the low pH of the cell-free cultural liquids but not due to the synthesis of bacteriocins. The combination of the genes of plantaricin regulon of L. plantarum ONU 206 and L. plantarum ONU 991 resembled that in L. plantarum J23 described in literature.Документ Effect of Bacillus megaterium ONU 500 on the germination and growth of sunflower seedlings(Одеський національний університет імені І. І. Мечникова, 2021) Shvets, Y. A.; Krylova, K. D.; Limanska, Nataliia V.; Швець, Ю. А.; Крилова, К. Д.; Ліманська, Наталія Вікторівна; Швец, Ю. А.; Крылова, Е. Д.; Лиманская, Наталия ВикторовнаThe aim of the study was to evaluate the effect of Bacillus megaterium ONU 500 on the germination and growth of sunflower seedlings. Materials and methods. Sunflower seeds were sown in containers with soil inoculated with a suspension of bacteria B. megaterium ONU 500 in concentration of 106 CFU/ml. After 21 days growth characteristics of seedlings were compared: germination, average height, root length, leaf area, dry weight. Effect of these microorganisms on biofilm formation on roots was investigated with light microscopy. Results. Inoculation of soil with B. megaterium ONU 500 resulted in the positive effect on roots length (increase by 21%), leaf area (increase by 22%) and dry weight of seedlings (increase by 17.8%). No significant effect on germination and plant height was observed. On the roots of plants grown in soil treated with B. megaterium ONU 500 more developed biofilms with well formed matrix were observed, which indicates the stimulation of ability of plant and soil microbiota to form biofilms in presence of bacilli from the studied strain. Conclusion. The investigations demonstrated that bacteria of B. megaterium ONU 500 strain caused a positive effect on development of sunflower seedlings and formation of biofilms on their roots.Документ EFFECT OF ENTEROCOCCUS DURANS BACTERIOCIN ON BACTERIAL WILT AGENT(Одеський національний університет імені І. І. Мечникова, 2012) Limanska, Nataliia V.; Choiset, Y.; Korotaieva, Nadiia V.; Sergeeva, Zh.; Chobert, J.-M.; Ivanytsia, Tetіana V.; Haertle, T.; Ліманська, Наталія Вікторівна; Іваниця, Тетяна Володимирівна; Шуазе, І.; Коротаєва, Надія Володимирівна; Сергєєва, Жанна Юріївна; Шобер, Ж.-М.; Іваниця, Володимир Олексійович; Ертле, Т.; Лиманская, Наталия Викторовна; Иваница, Татьяна Владимировна; Шуазе, И.; Коротаева, Надежда Владимировна; Сергеева, Жанна Юрьевна; Иваница, Владимир Алексеевич; Эртле, Т.; Ivanytsia, Volodymyr O.Effect of a bacteriocin produced by Enterococcus durans A5-11 on growth of Ralstonia solanacearum strains and their ability to cause wilt in tomatoes was investigated. Among tested phytopathogenic strains, 33% were sensitive and inhibited as it was shown after spotting bacteriocin on Ralstonia lawns. Minimal inhibitory concentrations and sizes of lysis or inhibition zones varied depending on the strain used for study. The same differences were evidenced for the effect of the bacteriocin on Ralstonia in a liquid medium and when inoculating Lycopersicon esculentum Mill test plants. Treatment of plant roots with the bacteriocin of E. durans A5-11 simultaneously with inoculation with bacteria of a highly susceptible strain R. solanacearum 6189 resulted in diminished number of wilted tomatoesДокумент Effect of lactobacillus plantarum and bacillus atrophaeus on growth of wheat seedlings(Одеський національний університет імені І. І. Мечникова, 2017) Zhunko, Inna D.; Merlich, Andrii H.; Limanska, Nataliia V.; Жунько, Інна Давидівна; Мерліч, Андрій Геннадійович; Ліманська, Наталія ВікторівнаThe effect of bacteria of the strains Lactobacillus plantarum ONU 12, ONU 311 and Bacillus atrophaeus ONU 528 on growth characteristics of wheat seedlings have been studied. Inoculation of surface sterilized seeds with the diluted cultures was conducted. The inoculated seeds were brought into wet-chambers, and after germination the measurements of seedling growth characteristics were done. The treatment by L. plantarum ONU 311 resulted in increase of the length of seedlings roots in 31% as compared with the control and stimulation effect on the height of seedlings was 48%. The mixture of the strains L. plantarum ONU 12 and ONU 311 increased the length of roots and height of seedlings in 41,1% and 52,4% accordingly. The treatment of wheat seeds by bacteria B. atrophaeus ONU 528 resulted in increase of mean length of roots in 39,7% and height of seedlings in 45% as compared with the control. Bacteria L. plantarum, B. atrophaeus and their mixtures had the stimulation effect both on roots and height of wheat seedlings.Документ Effect of Lactobacillus plantarum on agent of damping-off of pine seedlings(Одеський національний університет імені І. І. Мечникова, 2018) Adarma, N. Yu.; Kononiuk, Yu. V.; Limanska, Nataliia V.; Ліманська, Наталія ВікторівнаEffect of some Lactobacillus strains and their combinations on agents of fusariosis of pine Fusarium sp. 17 has been studied. Bacteria of L. plantarum species could be studied as perspective microorganisms for improving germination of Scots pine seeds and protection of seedlings under the certain condition of soil treatment.Документ Effect of Lactobacillus plantarum on germination and some growth characteristics of wheat(Odessa I. I. Mechnikova National University, 2017) Tverdokhlib, V. S.; Sokolova, N. V.; Sudak, A. A.; Limanska, Nataliia V.; Ліманська, Наталія ВікторівнаL. plantarum ONU 12 and L. plantarum ONU 311 positively effected on morphological characteristics of wheat seedlings germinated from seeds in a gel Aquasave S. Treatments of seeds with 1% of overnight cultures of these strains increased mean lengths of roots of seedlings in 72,2 - 75,0% and mean heights of the plants in 44-48,8%.Документ Effect of Lactobacillus plantarum on growth characteristics of wheat in hydroponics and soil(Одеський національний університет імені І. І. Мечникова, 2018) Limanska, Nataliia V.; Sokolova, N. V.; Sudak, A. A.; Galkin, Mykola B.; Ivanytsia, Volodymyr O.; Ліманська, Наталія Вікторівна; Соколова, Н. В.; Судак, А. А.; Галкін, Микола Борисович; Іваниця, Володимир Олексійович; Лиманская, Наталия Викторовна; Галкин, Николай Борисович; Иваница, Владимир АлексеевичThe aim was to study the effect of Lactobacillus plantarum on germination and some growth characteristics of wheat Triticum aestivum L. in hydroponics and soil under greenhouse and field conditions. Materials and Methods. Strains of L. plantarum, their mixtures in a ratio 1:1 had been prepared immediately before the experiment, as well as mixed cultures of these strains which had been cultivated together for at least one week and re-inoculated every two days were used. Before grounding, seeds were inoculated with lactobacilli (in concentrations 1%, 0.1%, 0.01%, 0.001%, 0.0001%, 0.00001%) for an hour. Seed germination, length of the roots and height of the seedlings were calculated as means with standard errors or confidential intervals. Results. Treatment of wheat seeds with individual L. plantarum ONU 12, L. plantarum ONU 311, L. plantarum ONU 355 strains, their mixed cultures grown together and mixtures has increased seed germination in 6.0–40.0% depending on the conditions of germination and inoculum concentration. Under conditions of hydroponics and soil in the greenhouse the best results were shown by inoculums in concentration 103–106 CFU/ml, and under field conditions – in concentration 102–104 CFU/ml. Mixtures and mixed cultures of L. plantarum ONU 12 + L. plantarum ONU 311 and L. plantarum ONU 12 + L. plantarum ONU 355 formed developed biofilms on roots of wheat seedlings. Mean height of seedlings increased in 8.0–41.0%, and the length of the roots increased by 2.4 times in hydroponics and in 6.8 –64.5% in soil. Conclusion. The mixtures and mixed cultures of bacteria L. plantarum ONU 12 + L. plantarum ONU 311 and L. plantarum ONU 12 + L. plantarum ONU 355 could stimulate wheat germination and growth and can be used for development of plant growth promoting bacterial preparations.Документ Effect of Lactobacillus plantarum on growth of some ornamental plants(Одеський національний університет імені І. І. Мечникова, 2018) Zavgorodna, K.; Limanska, Nataliia V.; Ліманська, Наталія ВікторівнаMixture of strains L. plantarum ONU 12 and L. plantarum ONU 311 could improve growth of lawn grasses, clove and matthiola increasing root length and height of seedlings.