Purification of bacteriocin from Enterococcus italicus onu547 by reversed phase-high performance liquid chromatography

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dc.contributor.authorMerlich, Andrii H.
dc.contributor.authorМерліч, Андрій Геннадійович
dc.contributor.authorМерлич, Андрей Геннадиевич
dc.date.accessioned2017-10-04T11:48:00Z
dc.date.available2017-10-04T11:48:00Z
dc.date.issued2017
dc.description.abstractPre-purified fractions of bacteriocin from E. italicus ONU547 were subjected to further purification by RP-HPLC with various methods. Two types of analytical columns (RP C8 and C18), gradient and step elution methods were used for experiment. After using of both columns with gradient elution method on chromatograms two peaks were observed, which fraction exhibited antagonistic activity against L. sakei that could indicate the two component nature of the studied bacteriocin. The most effective was the RP-HPLC with C8 column performed by step elution method resulted in bacteriocin activity given the zone of inhibition with diameter 1,75 sm. The purified fraction can be used for further study and characterization of bacteriocin.uk
dc.identifier.citationInternational conference of young scientists "Modern problems of microbiology and biotechnology", Odesa, Ukraine, June 20-24, 2017uk
dc.identifier.urihttps://dspace.onu.edu.ua/handle/123456789/10924
dc.language.isoenuk
dc.publisherOdessa I. I. Mechnikova National Universityuk
dc.subjectbacteriocinuk
dc.subjectpurificationuk
dc.subjectEnterococcus italicusuk
dc.subjectreversed phase-high performance liquid chromatographyuk
dc.titlePurification of bacteriocin from Enterococcus italicus onu547 by reversed phase-high performance liquid chromatographyuk
dc.typeArticleuk
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