Preference of molecular markers for detecting allelic variants of gliadins

dc.contributor.authorPopovych, Yuliia A.
dc.contributor.authorBlagodarova, O. M.
dc.contributor.authorChebotar, Sabina V.
dc.contributor.authorПопович, Юлія Андріївна
dc.contributor.authorЧеботар, Сабіна Віталіївна
dc.date.accessioned2024-03-12T12:46:49Z
dc.date.available2024-03-12T12:46:49Z
dc.date.issued2023
dc.description.abstractThe main method of studying allelic variants of gliadins is electrophoresis in acidic PAGE, which actually shows the phenotype determined by the set of genes of the gliadin-encoding locus. This method is difficult to use, because peptides encoded by all gliadin-encoding loci are present on one lane of the gel and may overlap. Therefore, there is a need for molecular markers that would allow the determination of allelic variants of gliadins using PCR. This will help to use gliadins for marker-asissted selection, to identify allelic variants of gliadins even before obtaining grain suitable for electrophoresis of storage proteins. In this regard, the aim of study was to analyze polymorphism of Gli‑1 loci using allele-specific molecular markers and microsatellites and find suitable molecular markers for detecting allelic variants of gliadins by PCR method.uk_UA
dc.identifierUDС 575.17:575.113.2:633.34
dc.identifier.citationВісник Одеського національного університету = Odesa National University Heralduk_UA
dc.identifier.urihttps://dspace.onu.edu.ua/handle/123456789/37835
dc.language.isoenuk_UA
dc.publisherОдеський національний університет імені І. І. Мечниковаuk_UA
dc.relation.ispartofseries;Т. 28, вип. 2(53).
dc.subjectmolecular markersuk_UA
dc.subjectallelic variantsuk_UA
dc.subjectgliadinsuk_UA
dc.titlePreference of molecular markers for detecting allelic variants of gliadinsuk_UA
dc.typeArticleuk_UA
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