Preference of molecular markers for detecting allelic variants of gliadins
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Дата
2023
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Одеський національний університет імені І. І. Мечникова
Анотація
The main method of studying allelic variants of gliadins is electrophoresis in
acidic PAGE, which actually shows the phenotype determined by the set of genes of
the gliadin-encoding
locus. This method is difficult to use, because peptides encoded
by all gliadin-encoding
loci are present on one lane of the gel and may overlap.
Therefore, there is a need for molecular markers that would allow the determination
of allelic variants of gliadins using PCR. This will help to use gliadins for marker-asissted
selection, to identify allelic variants of gliadins even before obtaining grain
suitable for electrophoresis of storage proteins. In this regard, the aim of study was
to analyze polymorphism of Gli‑1 loci using allele-specific
molecular markers and
microsatellites and find suitable molecular markers for detecting allelic variants of
gliadins by PCR method.
Опис
Ключові слова
molecular markers, allelic variants, gliadins
Бібліографічний опис
Вісник Одеського національного університету = Odesa National University Herald