Biodiversity of yeast cultures isolated from littoral areas of the regional landscape park «Tiligulskiy»
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Дата
2014
Науковий керівник
Укладач
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Анотація
The identification of yeast cultures was done by polymerase chain reaction (PCR), using universal yeast primers. Following
this, the morphology of each yeast culture was examined. The biochemical properties of each of these yeast cultures were
determined during fermentation to carbohydrates (forming acid and gas), with the formation of hydrogen sulphide (H2S) gas and
sulphur dioxide (SO2). Each yeast culture was identified according to its fermentation to the following carbohydrates: glucose,
fructose, sucrose, maltose, lactose, galactose, sorbitol, mannitol, and xylose. The yeast cultures that were predominantly isolated
from the Tiligul Estuary (benthic soils) littoral waters included: Candida albicans, Candida glabrata, Candida parapsilosis,
Candida tropicalis, Rhodotorula rubra, Saccharomyces cerevisiae. The yeast cultures that were predominantly isolated and
identified by PCR from the different grape varieties growing in the Tiligul estuary coastal area, following fermentation, included:
Metschnikowia aff. fructicola, Pichia barkeri, Pichia guilliermondii, Pichia kudriavzevii, Pichia sp., Candida albicans, Candida
tropicalis, Saccharomyces cerevisiae, Schizosaccharomyces pombe. Yeast populations were collected from the surface of grape
cultivars during harvesting season. Grapes were collected from Tiligul estuary coastal areas that included: the «Koblevo» and
«Leninka» vineyards, and around the Chernvono-Ukrainka villages. Subsequently, a wide biodiversity of yeast species were
shown to exist on the surfaces of ripe grapes obtained during harvesting season in coastal areas of the «Tiligulskiy» landscape
park. Some of the isolated and identified yeast strains had the potential for biotechnological use in the wine industry. These
included: Saccharomyces cerevisiae, and Schizosaccharomyces pombe. These yeast strains are currently in use in the wine
industry, and are authorised for use in the food industry. Other yeast strains had potential for use as reference yeast strains, which
could be used for testing sensitivity to anti-fungal agents, including: anti-fungal antibiotics and «azole» group agents.
Опис
Ключові слова
Saccharomyces cerevisiae, Schizosaccharomyces pombe, biodiversity of yeast, biotechnology of yeast, landscape park
Бібліографічний опис
Экосистемы, их оптимизация и охрана. - Симферополь: ТНУ, 2014.