Бацили Чорного Моря як джерело нових антибіотичних речовин
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Дата
2021
Науковий керівник
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Видавець
Одеський національний університет імені І. І. Мечникова
Анотація
Експериментальну частину роботи присвячено розробці біотехнології отримання антимікробних сполук зі спороутворюючих бактерій – мешканців Чорного моря.
Визначено антагоністичну активність 27 культур бактерій, що належать до 2 родів спороутворюючих бактерій – Bacillus (5 видів) та Paenibacillus (1 вид) щодо Staphylococcus aureus АТСС 25923, Escherichia coli АТСС 25922 та Candida albicans ATCC 18804, за культивування на середовищах Гаузе 1 та МПА за різних температурних режимів. Найвищу чутливість до антимікробних сполук досліджуваних штамів виявлено у культури S. aureus АТСС 25923 Оптимальними умовами для синтезу метаболітів, активних щодо грампозитивних бактерій, було культивування на середовищі Гаузе 1 за температури 30 °С. Активність щодо E. сoli АТСС 25922 була вищою на середовищі Гаузе 1, вплив температури був штамоспецифічним. Синтез метаболітів з антифунгальними властивостями відбувався більш активно на середовищі МПА, температурний режим на цей процес не впливав. Серед досліджуваних культур не виявлено антагоністу всіх тест-штамів. У результаті роботи розроблено біотехнологію отримання антимікробних сполук зі спороутворюючих бактерій Чорного моря.
Курсову роботу “Бацили Чорного Моря як джерело нових антибіотичних речовин” викладено на 43 сторінках друкованого тексту. В роботі наведено посилання нa 14 публікацій кирилицею та 30 латиницею.
The experimental part of the work is devoted to the development of biotechnology for the production of antimicrobial compounds from spore-forming bacteria - inhabitants of the Black Sea. Antagonistic activity of 27 cultures of bacteria belonging to 2 genera of spore-forming bacteria - Bacillus (5 species) and Paenibacillus (1 species) against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Candida albicans ATCC 18804, was determined at different temperatures. The highest sensitivity to antimicrobial compounds of the studied strains was found in the culture of S. aureus ATCC 25923 The optimal conditions for the synthesis of metabolites active against gram-positive bacteria was cultivation on Gause medium 1 at a temperature of 30 ° C. The activity against E. coli ATCC 25922 was higher on Gause medium 1, the effect of temperature was strain-specific. The synthesis of metabolites with antifungal properties was more active on the Nutrient agar medium, the temperature regime did not affect this process. No antagonist of all test strains was detected among the studied cultures. As a result, biotechnology for the production of antimicrobial compounds from spore-forming bacteria of the Black Sea was developed. The course work "Bacilli of the Black Sea as a source of new antibiotic substances" is set out on 43 pages of printed text. The paper provides references to 14 publications in Cyrillic and 30 in Latin.
A comparative analysis of the effectiveness of the use of test systems "RealBest DNA extraction-3" and "Licking solution" to optimize the diagnosis of papillomavirus infection by molecular biotechnological PCR method. Comparative analysis of the effectiveness of the two test systems showed the high specificity and sensitivity of the system "RealBest DNA extraction-3". It can be recommended as optimal for DNA isolation in the laboratory diagnosis of human papilloma virus infection. We also compared the efficiency of human papillomavirus DNA isolation from two types of biological material. It was found that the efficiency of DNA isolation is greater in urogenital scrapings. The work is presented on 46 pages, it contains 4 tables and 4 figures. References are made to 51 sources of literature (34 in Cyrillic and 17 in Latin).
The experimental part of the work is devoted to the development of biotechnology for the production of antimicrobial compounds from spore-forming bacteria - inhabitants of the Black Sea. Antagonistic activity of 27 cultures of bacteria belonging to 2 genera of spore-forming bacteria - Bacillus (5 species) and Paenibacillus (1 species) against Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922 and Candida albicans ATCC 18804, was determined at different temperatures. The highest sensitivity to antimicrobial compounds of the studied strains was found in the culture of S. aureus ATCC 25923 The optimal conditions for the synthesis of metabolites active against gram-positive bacteria was cultivation on Gause medium 1 at a temperature of 30 ° C. The activity against E. coli ATCC 25922 was higher on Gause medium 1, the effect of temperature was strain-specific. The synthesis of metabolites with antifungal properties was more active on the Nutrient agar medium, the temperature regime did not affect this process. No antagonist of all test strains was detected among the studied cultures. As a result, biotechnology for the production of antimicrobial compounds from spore-forming bacteria of the Black Sea was developed. The course work "Bacilli of the Black Sea as a source of new antibiotic substances" is set out on 43 pages of printed text. The paper provides references to 14 publications in Cyrillic and 30 in Latin.
A comparative analysis of the effectiveness of the use of test systems "RealBest DNA extraction-3" and "Licking solution" to optimize the diagnosis of papillomavirus infection by molecular biotechnological PCR method. Comparative analysis of the effectiveness of the two test systems showed the high specificity and sensitivity of the system "RealBest DNA extraction-3". It can be recommended as optimal for DNA isolation in the laboratory diagnosis of human papilloma virus infection. We also compared the efficiency of human papillomavirus DNA isolation from two types of biological material. It was found that the efficiency of DNA isolation is greater in urogenital scrapings. The work is presented on 46 pages, it contains 4 tables and 4 figures. References are made to 51 sources of literature (34 in Cyrillic and 17 in Latin).
Опис
Ключові слова
162 біотехнології та біоінженерія, спороутворюючі бактерії, антимікробні метаболіти, умовно-патогенні мікроорганізми, spore-forming bacteria, antimicrobial metabolites, opportunistic pathogens
Бібліографічний опис
Болдирєва, В. В. Бацили Чорного моря як джерело нових антибіотичних речовин = Bacilli of the Black Sea as a sourse of new antibiotic substances : Дипломна робота бакалавра / В. В. Болдирєва. – Одеса, 2021. – 46 с.