Fatty acids composition of Bacillus subtilis ONU551 lipids
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Дата
2019
Науковий керівник
Укладач
Редактор
Назва журналу
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Назва тому
Видавець
Анотація
The aim of the study was to determine the cellular lipids fatty acid composition for identification of
the Bacillus subtilis ONU 551 strain bacteria, which is a phenol destructor. Fatty acids analysis of B. subtilis
ONU 551 strain was performed using an automatic system for microorganisms’ identification MIDI Sherlock
(MIDI , USA ) based on gas chromatograph Agilent 7890. Chromatograms analysis showed that the fatty acid
spectrum of the strain B. subtilis ONU 551 consisted predominately of branched structural isomers of saturated
acids: 13-methyltetradecanoic (15:0 iso; 34.72%) and 12-methyltetradecanoic (15:0 anteiso; 33.72%)
acids. The total content of the branched saturated fatty acids was 88.16% – 14:0 iso (0.52%), 15:0 iso (34.72%),
15:0 anteiso (33.72%), 16:0 iso (1.85%), 17:0 iso (7.11%), 17:0 anteiso (10.24%). The saturated fatty acids of the
normal structure were also detected – 12:0 (0.36%), 14:0 (0.28%), 16:0 (1.30%). No 2- and 3-hydroxy acids
and no cyclic fatty acids were detected in the fatty acid profile of B. subtilis ONU 551 strain. Unsaturated fatty
acid isomers – 15:1 w5c (1.85%), 16:1 w11c (1.21%), 16:1 w7c alcohol (1.08%), 17:1 iso w10c (3.18%), Σ17:1 iso
I/anteiso B (2.57%) were shown to be the distinctive biomarkers of the B. subtilis ONU 551 strain. According to
the fatty acid profile analysis with MIDI Sherlock system, the studied strain was identified as Bacillus subtilis
with high level of similarity index (0.563).
Опис
Ключові слова
Bacillus subtilis ONU 551, fatty acids composition, saturated acids isomers, strain identification
Бібліографічний опис
The Ukrainian biochemical journal