Cellular fatty acid composition of Aeromonas genus – destructor of aromatic xenobiotics
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Дата
2019
Науковий керівник
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Анотація
The aim of this study was a determination of the fatty acid composition of cellular lipids and identification
of the strains, isolated from the wastewater of pharmaceutical production, – the destructor of aromatic
xenobiotics. The phenotypic characteristics and cellular fatty acid (FA) composition confirmed the strain
belonging to the Aeromonas ichthiosmia with the similarity index of library data MIDI Sherlock - 0.564.
Analysis of the cellular FA composition of the strain Aeromonas ichthiosmia ONU 552 was carried out using
the MIDI Sherlock microorganism identification system based on the gas chromatograph Agilent 7890. Chromatographic
analysis showed that the fatty acid profile of the strain Aeromonas ichthiosmia ONU 552 contains
26 fatty acids with the total number of carbon atoms from 10 to 18. 85.27% of saturated and unsaturated fatty
acids had unbranched structure. The total content of unsaturated fatty acids – 16:1 w7c/16:1 w6c, 18:1 w7c,
16:1 w7c alcohol, 17:1 w8c, 17:1 w6c, 16:1 w5c, was 50% of the total fatty acid pool. Less than 1.5% branched
fatty acids were predominantly in the iso form: 13:0 iso (0.20%); 15:0 iso (0.97%); 17:1 iso w9c (1.35%), 17:0
iso (1.49%); in the anteiso form, only one acid 17:0 (0.27%) was identified. It was shown that the characteristic
of the fatty acid composition of the strain Aeromonas ichthiosmia ONU 552 – the destructor of aromatic
xenobiotics,
was the presence of hydroxyacids 12:0 3OH, 15:0 3OH, 15:0 iso 3OH and dominance of hexadecanoic
(16:0) and hexadecenoic (16:1 w7c/16:1 w6c) of fatty acids.
Опис
Ключові слова
Aeromonas ichthiosmia, destructor of aromatic xenobiotics, cellular fatty acid composition
Бібліографічний опис
The Ukrainian biochemical journal