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dc.contributor.authorGudzenko, Tetiana V.-
dc.contributor.authorVoliuvach, Olha V.-
dc.contributor.authorHorshkova, Olena H.-
dc.contributor.authorOstapchuk, Andriy M.-
dc.contributor.authorIvanytsia, Volodymyr O.-
dc.contributor.authorГудзенко, Тетяна Василівна-
dc.contributor.authorВолювач, Ольга В'ячеславівна-
dc.contributor.authorГоршкова, Олена Георгіївна-
dc.contributor.authorІваниця, Володимир Олексійович-
dc.contributor.authorГудзенко, Татьяна Васильевна-
dc.contributor.authorВолювач, Ольга Вячеславовна-
dc.contributor.authorГоршкова, Елена Георгиевна-
dc.contributor.authorИваница, Владимир Алексеевич-
dc.identifierUDC 531/534/: [57+61]-
dc.identifier.citationThe Ukrainian biochemical journaluk_UA
dc.description.abstractBiotechnological treatment of enterprises wastewater, which contains toxic phenolic compounds, is the most acceptable method today. Therefore, the search for new non-pathogenic biochemically active microorganisms - destructors of phenol and their identification is relevant. The aim of this study was to determine phenol-oxidizing activity and the fatty acid profile of Brevibacillus centrosporus F14 – destructor of phenol, isolated from pharmaceutical plant wastewater. Fatty acids analysis of the microorganism culture was carried out using automatic system of microorganisms identification MIDI Sherlock based on gas chromatograph Agilent 7890. The phenol-oxidizing activity of bacteria was determined by photometric method according to the degree of phenol extraction from the water. It was experimentally confirmed that Brevibacillus sp. F14 strain had a phenol-destructive activity. When phenol-containing water was treated with Brevibacillus sp. F14 cells, phenol concentration in the water decreased from 200.0 ± 12.0 mg/l to 6.8 ± 0.8 mg/l during 15 days of exposure. Chromatographic analysis showed that saturated fatty acids i-14:0 (14.9%), i-15:0 (14.8%), a-15:0 (34.9%), i-16:0 (11.10%) dominated in the strain’s fatty acids profile. The total content of the branched saturated fatty acids i-14:0, i-15:0, a-15: 0, i-16: 0, i-17: 0, a-17:0, Σ(a-17:1/i-17:1) was 85.0% and that of saturated and unsaturated fatty acids with the normal structure – 7% of the total fatty acids pool. The distinction of Brevibacillus centrosporus F14 strain’s fatty acid profile from other bacteria of Brevibacillus genus was the presence of more i-14:0 (14.85%), i-16:0 (11.10%) fatty acids and 16: 1 w7c alcohol (7.71%), as well as the minor content of fatty acids markers 16:0 N alcohol (0.60%), 18:3 w6c (0.4%) and Σ(a-17:1/i-17:1) (1.94%). According to the phenotypic characteristics and fatty acid composition, the tested phenol-oxidizing strain belongs to Brevibacillus centrosporus species. The index of Brevibacillus centrosporus F14 strain’s fatty acid profile similarity with the library was 0.645.uk_UA
dc.relation.ispartofseries;Vol. 92, № 1.-
dc.subjectBrevibacillus centrosporusuk_UA
dc.subjectdestructor of phenoluk_UA
dc.subjectcomposition of fatty acidsuk_UA
dc.titlePhenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strainuk_UA
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