Phenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strain
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Дата
2020
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Анотація
Biotechnological treatment of enterprises wastewater, which contains toxic phenolic compounds, is
the most acceptable method today. Therefore, the search for new non-pathogenic biochemically active microorganisms
- destructors of phenol and their identification is relevant. The aim of this study was to determine
phenol-oxidizing activity and the fatty acid profile of Brevibacillus centrosporus F14 – destructor of phenol,
isolated from pharmaceutical plant wastewater. Fatty acids analysis of the microorganism culture was carried
out using automatic system of microorganisms identification MIDI Sherlock based on gas chromatograph
Agilent 7890. The phenol-oxidizing activity of bacteria was determined by photometric method according
to the degree of phenol extraction from the water. It was experimentally confirmed that Brevibacillus sp.
F14 strain had a phenol-destructive activity. When phenol-containing water was treated with Brevibacillus
sp. F14 cells, phenol concentration in the water decreased from 200.0 ± 12.0 mg/l to 6.8 ± 0.8 mg/l during 15
days of exposure. Chromatographic analysis showed that saturated fatty acids i-14:0 (14.9%), i-15:0 (14.8%),
a-15:0 (34.9%), i-16:0 (11.10%) dominated in the strain’s fatty acids profile. The total content of the branched
saturated fatty acids i-14:0, i-15:0, a-15: 0, i-16: 0, i-17: 0, a-17:0, Σ(a-17:1/i-17:1) was 85.0% and that of saturated
and unsaturated fatty acids with the normal structure – 7% of the total fatty acids pool. The distinction
of Brevibacillus centrosporus F14 strain’s fatty acid profile from other bacteria of Brevibacillus genus was
the presence of more i-14:0 (14.85%), i-16:0 (11.10%) fatty acids and 16: 1 w7c alcohol (7.71%), as well as the
minor content of fatty acids markers 16:0 N alcohol (0.60%), 18:3 w6c (0.4%) and Σ(a-17:1/i-17:1) (1.94%).
According to the phenotypic characteristics and fatty acid composition, the tested phenol-oxidizing strain
belongs to Brevibacillus centrosporus species. The index of Brevibacillus centrosporus F14 strain’s fatty acid
profile similarity with the library was 0.645.
Опис
Ключові слова
Brevibacillus centrosporus, destructor of phenol, identification, composition of fatty acids
Бібліографічний опис
The Ukrainian biochemical journal