Порівняльна ефективність препаратів біофлавоноїдів при експериметальному гепатиті
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Дата
2019
Науковий керівник
Укладач
Редактор
Назва журналу
ISSN
2077-1746
E-ISSN
2415-3125
Назва тому
Видавець
Одеський національний університет імені І. І. Мечникова
Анотація
Проведено експериментальне дослідження гепатопротекторної та антидисбіотичної дії нових вітчизняних препаратів з натуральної сировини на моделі токсичного гепатиту у щурів. Препарат Лекасил з вмістом лецитину та флаволігнанів розторопши має більш виражену гепатопротекторну дію, а Квертулін, який складається з кверцетину та інуліну, більш істотно знижує ступінь дисбіозу в слизовій оболонці товстої кишки щурів з гепатитом.
The aim of the work is comparative study of hepatoprotective properties of Quertulin (quercetin, inulin and calcium citrate) and Lecasil (squeezed from leaves of thistle, lecithin and calcium citrate) in rats on the background of toxic hepatitis modeling. Materials and Methods: The studies were conducted on 28 white rats, with caused toxic hepatitis. Prophylaxis of the disease was performed using Quertulin or Leсasil (300 mg/kg) from the first day of the experiment. Toxic hepatitis was reproduced by intra-abdominal administration of hydrazine sulfate (50 mg/kg) at 8, 9 and 10 days. The duration of the experiment was 14 days. In liver homogenates, inflammation markers and cholestasis were determined – the activity of elastase and alkaline phosphatase. In the mucous membrane of the colon the determination of a marker of microbial contamination – activity of urease and non-specific antimicrobial factor – activity of lysozyme was carried out. In the serum, the determination of "liver" markers was performed, the activity of the alanine aminotransferase of alkaline phosphatase and the content of bilirubin. Results: Preventive use of drugs Lecasil and Quertulin effectively prevents metabolic disorders of the hepatobiliary system: reduced bilirubin levels by 20 and 28%, ALT activity – by 57 and 43% and alkaline phosphatase activity – by 49 and 17%, respectively. The studied drugs also reduced the development of dysbiosis, formed in the colon of rats under conditions of toxic hepatitis: Quertulin reduced the degree of dysbiosis by 32.2%, and Leсasil by 15.6%. Conclusion: The effectiveness of new domestic drugs in the model of toxic hepatitis in rats is shown. According to the results obtained, the more pronounced hepatoprotective effect is propuced by the dietary supplement of Lecasil, and more significant reduction of the dysbiosis degree in the mucous membrane of the colon of rats with hepatitis – by Quertulin. The application of the studied dietary supplements provides effective hepatoprotective and anti-dysbiosis action in the colon of rats with toxic hepatitis.
The aim of the work is comparative study of hepatoprotective properties of Quertulin (quercetin, inulin and calcium citrate) and Lecasil (squeezed from leaves of thistle, lecithin and calcium citrate) in rats on the background of toxic hepatitis modeling. Materials and Methods: The studies were conducted on 28 white rats, with caused toxic hepatitis. Prophylaxis of the disease was performed using Quertulin or Leсasil (300 mg/kg) from the first day of the experiment. Toxic hepatitis was reproduced by intra-abdominal administration of hydrazine sulfate (50 mg/kg) at 8, 9 and 10 days. The duration of the experiment was 14 days. In liver homogenates, inflammation markers and cholestasis were determined – the activity of elastase and alkaline phosphatase. In the mucous membrane of the colon the determination of a marker of microbial contamination – activity of urease and non-specific antimicrobial factor – activity of lysozyme was carried out. In the serum, the determination of "liver" markers was performed, the activity of the alanine aminotransferase of alkaline phosphatase and the content of bilirubin. Results: Preventive use of drugs Lecasil and Quertulin effectively prevents metabolic disorders of the hepatobiliary system: reduced bilirubin levels by 20 and 28%, ALT activity – by 57 and 43% and alkaline phosphatase activity – by 49 and 17%, respectively. The studied drugs also reduced the development of dysbiosis, formed in the colon of rats under conditions of toxic hepatitis: Quertulin reduced the degree of dysbiosis by 32.2%, and Leсasil by 15.6%. Conclusion: The effectiveness of new domestic drugs in the model of toxic hepatitis in rats is shown. According to the results obtained, the more pronounced hepatoprotective effect is propuced by the dietary supplement of Lecasil, and more significant reduction of the dysbiosis degree in the mucous membrane of the colon of rats with hepatitis – by Quertulin. The application of the studied dietary supplements provides effective hepatoprotective and anti-dysbiosis action in the colon of rats with toxic hepatitis.
Опис
Ключові слова
щури, токсичний гепатит, дисбіоз, профілактика захворювань гепатобіліарної системи, rats, toxic hepatitis, dysbiosis, prevention of diseases of the hepatobiliary system
Бібліографічний опис
Макаренко О. А. Порівняльна ефективність препаратів біофлавоноїдів при експериметальному гепатиті / О. А. Макаренко, Т. В. Могілевська // Вісник Одеського національного університету. Біологія. – 2019. – Т. 24, вип. 2(45). – С. 129–135.
ORCID:
УДК
616.36:616.3:615.015