The spread of grapevine viruses on the south Ukraine

Abstract

Screening investigations of 726 grapevine samples for the presence of grapevine leafroll-associated viruses (GLRaV-1 and GLRaV-3) grapevine fleck virus (GFkV), grapevine fanleaf virus (GFLV), grapevine virus A (GVA), grapevine virus B (GVB), Rupestris stem pitting associated virus (RSPaV) have been conducted. The grapevine material selected from vineyards of the southern regions of Ukraine was tested by enzyme-linked immunosorbent assay (ELISA-test) and reverse transcription - polymerase chain reaction (RT-PCR). Using both these methods data about the spread of latent viral infections on the vineyards were obtained. The protocol of polymerase chain reaction was optimized during the investigation. GLRaV 1, GLRaV 3, GFLV and GFkV were revealed. Our data indicate the necessity of grapevine planting material certification in Ukraine.