Комп ’ютерне моделювання просторової структури білка - модулятора гіберелінової відповіді RHT-1 Triticum Aestivum L. з родини Della -Gras білків
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Дата
2016
Науковий керівник
Укладач
Редактор
Назва журналу
ISSN
E-ISSN
Назва тому
Видавець
Анотація
Modeling of 3D structure of wheat RHT-1 protein (Reduced height protein 1, Ta-RHT1) which is a modulator
of plant growth hormone gibberellin response and belongs to the DELLA subfamily of GRAS family of plant protein
repressors of transcription. Methods. BLAST-analysis, 3D structure modeling by Robetta, I-TASSER and Swiss-Model
web-servers. Results. For Ta-RHT1 protein the secondary and subdomain structure and regions of high structural
disorder are predicted. Crystal structures of SAM-dependent methyltransferases containing β-layer with seven β-strands
were used as structural templates for modeling. Conservative tyrosine Y594 residue in SAW-domain can probably be
phosphorylated and ensure the regulation of activity of Ta-RHT1 protein. N-terminal deletion M1-E64 in Ta-RHT1 is
associated with altered response to growth hormone gibberellin and therefore shorter stalk of wheat and its resistance
to lodging, leads to loss of specific 38DELLA42 and 60LExLE64 motifs. Since 38DELLA42 motif is required for binding to
gibberellin receptor, apparently these mutations resulted in violations of specific binding of Ta-RHT1 with GID1 and
violation of the subsequent proteolysis and ubiquitination of DELLA-protein.
Опис
Ключові слова
DELLA protein, GRAS family, gibberellin response, structure modeling
Бібліографічний опис
Фактори експериментальної еволюції організмів.