Purification of bacteriocin from Enterococcus italicus onu547 by reversed phase-high performance liquid chromatography
Вантажиться...
Дата
2017
Науковий керівник
Укладач
Редактор
Назва журналу
Номер ISSN
Номер E-ISSN
Назва тому
Видавець
Odessa I. I. Mechnikova National University
Анотація
Pre-purified fractions of bacteriocin from E. italicus ONU547 were
subjected to further purification by RP-HPLC with various methods. Two
types of analytical columns (RP C8 and C18), gradient and step elution
methods were used for experiment. After using of both columns with
gradient elution method on chromatograms two peaks were observed,
which fraction exhibited antagonistic activity against L. sakei that could
indicate the two component nature of the studied bacteriocin. The most
effective was the RP-HPLC with C8 column performed by step elution
method resulted in bacteriocin activity given the zone of inhibition with
diameter 1,75 sm. The purified fraction can be used for further study and
characterization of bacteriocin.
Опис
Ключові слова
bacteriocin, purification, Enterococcus italicus, reversed phase-high performance liquid chromatography
Бібліографічний опис
International conference of young scientists "Modern problems of microbiology and biotechnology", Odesa, Ukraine, June 20-24, 2017