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Title: Purification of bacteriocin from Enterococcus italicus onu547 by reversed phase-high performance liquid chromatography
Authors: Merlich, Andrii H.
Мерліч, Андрій Геннадійович
Мерлич, Андрей Геннадиевич
Citation: International conference of young scientists "Modern problems of microbiology and biotechnology", Odesa, Ukraine, June 20-24, 2017
Issue Date: 2017
Publisher: Odessa I. I. Mechnikova National University
Keywords: bacteriocin
Enterococcus italicus
reversed phase-high performance liquid chromatography
Abstract: Pre-purified fractions of bacteriocin from E. italicus ONU547 were subjected to further purification by RP-HPLC with various methods. Two types of analytical columns (RP C8 and C18), gradient and step elution methods were used for experiment. After using of both columns with gradient elution method on chromatograms two peaks were observed, which fraction exhibited antagonistic activity against L. sakei that could indicate the two component nature of the studied bacteriocin. The most effective was the RP-HPLC with C8 column performed by step elution method resulted in bacteriocin activity given the zone of inhibition with diameter 1,75 sm. The purified fraction can be used for further study and characterization of bacteriocin.
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