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Title: Biodestruction of phenol by non-pathogenic bacteria of the genus Pseudomonas
Authors: Horshkova, Olena H.
Voliuvach, Olha V.
Bebyk, V. V.
Kolomiets, O. G.
Lagovskaya, L. S.
Khadzhy, V. D.
Волювач, Ольга Вячеславовна
Волювач, Ольга В'ячеславівна
Горшкова, Олена Георгіївна
Горшкова, Елена Георгиевна
Citation: International conference of young scientists "Modern problems of microbiology and biotechnology", Odesa, Ukraine, June 20-24, 2017
Issue Date: 2017
Publisher: Odessa I. I. Mechnikova National University
Keywords: biodegradation
bacteria of the genus Pseudomonas
Abstract: It has been experimentally established that two strains of bacteria genus Pseudomonas, isolated from the marine environment and identified by the fatty acid composition of their cellular lipids as P. fluorescens ONU328, P. maltophilia ONU329, are not pathogenic and have a high phenol-oxidizing ability. Bacteria are cultured for two days at a temperature of 30 °C on MPA, further bacterial cells are suspended in the mineral medium M-9, and in the optimum amount is introduced into the contaminated water containing up to 300 mg/l phenol. It was established that the strain of P. maltophilia possessed a greater phenol-oxidizing activity than the strain P. fluorescens. When water was treated with strain P. maltophilia with a culture concentration of 3.5×104 CFU/ml, deep purification from phenol was observed on 34 day, and with an increase in the culture concentration to 7.5×105 CFU /ml on 18 day, t=30 °C.
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